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AKT2 antibody

KO/KD Overexpression
Anti-AKT2 antibody used in Western Blot (WB). GTX128457
Anti-AKT2 antibody used in Western Blot (WB). GTX128457
Anti-AKT2 antibody used in Western Blot (WB). GTX128457
Anti-AKT2 antibody used in Western Blot (WB). GTX128457
Anti-AKT2 antibody used in Western Blot (WB). GTX128457
Anti-AKT2 antibody used in Western Blot (WB). GTX128457

Cat. No. GTX128457

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all AKT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

Raw264.7 , C2C12 , PC-12 , HA-human AKT2-transfected 293T cells

Product Note

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Akt2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2750992

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 2 , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA

Background

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AKT2 antibody used in Western Blot (WB). GTX128457

GTX128457 WB Image

Akt2 antibody detects Akt2 protein by Western blot analysis.
A. 30 μg Raw264.7 whole cell lysate/extract
B. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Akt2 antibody (GTX128457) dilution: 1:500

Anti-AKT2 antibody used in Western Blot (WB). GTX128457

GTX128457 WB Image

Akt2 antibody detects Akt2 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
Akt2 antibody (GTX128457) dilution: 1:500

Anti-AKT2 antibody used in Western Blot (WB). GTX128457

GTX128457 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT2 antibody (GTX128457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT2 antibody used in Western Blot (WB). GTX128457

GTX128457 WB Image

Overexpression

Akt2 antibody detects Akt2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of HA-human Akt1-transfected 293T cells
C. 5 μg whole cell lysate/extract of HA-human Akt2-transfected 293T cells
7.5 % SDS-PAGE
Akt2 antibody (GTX128457) dilution: 1:20000

Anti-AKT2 antibody used in Western Blot (WB). GTX128457

GTX128457 WB Image

PG-4 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT2 antibody (GTX128457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT2 antibody used in Western Blot (WB). GTX128457

GTX128457 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT2 antibody (GTX128457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Zemanovic S et al. Cell Rep 2018; 25 (9) : 2605-2616.e7 Dynamic Phosphorylation of the C Terminus of Hsp70 Regulates the Mitochondrial Import of SOD2 and Redox Balance.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

AKT2 antibody Cat. No. GTX128457

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-AKT2 antibody used in Western Blot (WB). GTX128457
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells
Amount used : 50μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169