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Annexin A1 antibody

KO/KD Comparison Orthogonal Overexpression
Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070
Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070
Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070
Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070
Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070
Anti-Annexin A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101070
Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070
Anti-Annexin A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101070
Anti-Annexin A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101070
Anti-Annexin A1 antibody used in Immunoprecipitation (IP). GTX101070

Cat. No. GTX101070

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Annexin A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

NIH-3T3 , JC

Predict Reactivity

Rabbit, Bovine, Dog, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036236

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

annexin A1 , ANX1 , LPC1

Cellular Localization

Nucleus , Cytoplasm , Cell projection , cilium , Basolateral cell membrane

Background

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070

GTX101070 WB Image

KO/KD

Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070

GTX101070 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Annexin A1 antibody (GTX101070) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070

GTX101070 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070

GTX101070 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070

GTX101070 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Annexin A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101070

GTX101070 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 Xenograft , using Annexin I(GTX101070) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070

GTX101070 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101070

GTX101070 IHC-P Image

Annexin A1 antibody detects Annexin A1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Annexin A1 stained by Annexin A1 antibody (GTX101070) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Annexin A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101070

GTX101070 ICC/IF Image

Annexin A1 antibody detects Annexin A1 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Annexin A1 stained by Annexin A1 antibody (GTX101070) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Annexin A1 antibody used in Immunoprecipitation (IP). GTX101070

GTX101070 IP Image

Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 μg of Annexin A1 antibody (GTX101070).
Western blot analysis was performed using Annexin A1 antibody (GTX101070).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Jing Chen et al. Front Immunol 2022; 13 : 875412 HA/CD44 Regulates the T Helper 1 Cells Differentiation by Activating Annexin A1/Akt/mTOR Signaling to Drive the Pathogenesis of EAP.
Application : WB
Reactivity : Mouse
Santarsiero A et al. Oxid Med Cell Longev 2021; 2021 : 5533793 Phenolic Compounds of Red Wine Aglianico del Vulture Modulate the Functional Activity of Macrophages via Inhibition of NF-κB and the Citrate Pathway.
Application : WB
Reactivity : Human
Kuo CH et al. Cell Biochem Funct 2020; (Epub) Biomarker discovery in highly invasive lung cancer cell through proteomics approaches.
Application : WB
Reactivity : Human
Wang C et al. Environ Toxicol Pharmacol 2019; 72 : 103270 Common differentially expressed proteins were found in mouse cleft palate models induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and retinoic acid.
Application : WB
Reactivity : Mouse
Fan CY et al. Electrophoresis 2013; 34 (24) : 3305-14 Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169