CD44 antibody [GT462]

Name: CD44 antibody [GT462]
Brand: GeneTex
SKU: GTX628895
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895

Anti-CD44 antibody [GT462] used in Flow cytometry (FACS). GTX628895

Anti-CD44 antibody [GT462] used in IHC (Paraffin sections) (IHC-P). GTX628895

Cat. No. GTX628895

Reference 5

Host	Mouse
Clonality	Monoclonal
Clone Name	GT462
Isotype	IgG2b
Application	WB, IHC-P, FACS, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all CD44 products

View product citations for antibody GTX628895 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
FACS	1:50-1:200
IP	Assay dependent

Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

A431 , MDA-MB-231(24 μg/ml Tunicamycin treatment for 16 hr) , HeLa(12 μg/ml Tunicamycin treatment for 48 hr)

Product Note

This antibody was raised against human CD44 Extracellular domain.
IP/MS validation was supported by references (PMID:30377425)

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD44. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888071

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1

Cellular Localization

Membrane

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq]

Database

Gene ID: 960 CD44
UniProt: P16070 CD44

Research Area

DATA IMAGES

GTX628895 WB Image

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:500.

GTX628895 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000.
Multiple bands were observed 130~180 kDa. It is possibly due to alternative splicing.
Reference: PMID: 20962267

GTX628895 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.(br)Reference:PMID: 10050880

GTX628895 WB Image

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX628895 FACS Image

CD44 antibody detects CD44 protein by flow cytometry analysis.
Sample: HL-60 cell fixed in 4% paraformaldehyde at 4oC for 5 min.
Black: Isotype control dilution: 1:100.
Green: CD44 antibody (GTX628895) dilution: 1:100.

GTX628895 IHC-P Image

CD44 antibody [GT462] detects CD44 protein at membrane on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
CD44 antibody [GT462] (GTX628895) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX628895 IHC-P Image

CD44 antibody [GT462] detects CD44 protein at cell membrane by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human breast cancer.
CD44 stained by CD44 antibody [GT462] (GTX628895) diluted at 1:200.
Antigen Retrieval: EDTA buffer, 20 min

REFERENCE

Application Reference

Justyna Mazurkiewicz et al. Cell Commun Signal 2022; 20 (1) : 63 Melanoma cells with diverse invasive potential differentially induce the activation of normal human fibroblasts.

Application : WB

Reactivity : Human

Xu H et al. Tissue Eng Part A 2021; Stem cell-seeded 3D-printed scaffolds combined with self-assembling peptides for bone defect repair.

Application : FACS

Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.

Application : IP

Reactivity : Human

Kim MC et al. BMC Cancer 2018; 18 (1) : 819 Hypoxia promotes acquisition of aggressive phenotypes in human malignant mesothelioma.

Application : FACS

Reactivity : Human

Valarmathi MT et al. Tissue Eng Part A 2017; (Epub) Functional Tissue-Engineering: A Prevascularized Cardiac Muscle Construct for Validating hMSCs Engraftment Potential in Vitro.

Application : FACS

Reactivity : Human

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