Caspase 1 antibody [14F468]

Cat. No. GTX14367

Reference 8

Host	Mouse
Clonality	Monoclonal
Clone Name	14F468
Isotype	IgG1
Application	WB, IHC-P, FACS
Reactivity	Human, Mouse, Rat
Package	50 μg ($399)

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View product citations for antibody GTX14367 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.5 - 2 μg/ml
IHC-P	1:10 - 1:500
FACS	Assay dependent

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Product Note

The antibody will recognize full-length Caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein.

Predict Reactivity

Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human caspase-1 was used as immunogen.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_368949

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 1 , ICE , IL1BC , P45

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Gene ID: 834 CASP1
UniProt: P29466 CASP1

Research Area

DATA IMAGES

GTX14367 WB Image

WB analysis of HeLa (A) and NIH-3T3 (B) cell lysate using GTX14367 Caspase 1 antibody [14F468].
Dilution : 0.5 μg/ml and 2 μg/ml

GTX14367 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX14367 Caspase 1 antibody [14F468].
Dilution : 5 μg/ml

GTX14367 IHC-P Image

IHC-P analysis of human lung tissue using GTX14367 Caspase 1 antibody [14F468].
Dilution : 5 μg/ml

GTX14367 IHC-P Image

IHC-P analysis of human rectum adenocarcinoma tissue using GTX14367 Caspase 1 antibody [14F468].
Dilution : 5 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0