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Caspase 1 (cleaved Asp297) antibody

Comparison Orthogonal
Anti-Caspase 1 (cleaved Asp297) antibody used in Western Blot (WB). GTX133447
Anti-Caspase 1 (cleaved Asp297) antibody used in Western Blot (WB). GTX133447

Cat. No. GTX133447

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Caspase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

THP-1 conditioned medium (500 ng/ml LPS treatment for 4 hr in serum free and 1 mM ATP treatment for 1.5 hr)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 1 (cleaved Asp297) . The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886987

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 1 , ICE , IL1BC , P45

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-Caspase 1 (cleaved Asp297) antibody used in Western Blot (WB). GTX133447

GTX133447 WB Image

Orthogonal

Treated (+) THP-1 whole cell extracts and conditioned medium (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cleaved Caspase 1 (p20) antibody (GTX133447) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 1 (cleaved Asp297) antibody used in Western Blot (WB). GTX133447

GTX133447 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts and conditioned medium (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Caspase 1 (cleaved Asp297) antibody (GTX133447) diluted at 1:1000 and competitor's antibody (#4199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

Li H et al. J Inflamm Res 2021; 14 : 4839-4858 Neutrophil Extracellular Traps Augmented Alveolar Macrophage Pyroptosis via AIM2 Inflammasome Activation in LPS-Induced ALI/ARDS.
Application : WB
Pons BJ et al. Cell Mol Life Sci 2021; 78 (17-18) : 6319-6335 Chronic exposure to Cytolethal Distending Toxin (CDT) promotes a cGAS-dependent type I interferon response.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169