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ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody

Orthogonal
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819

Cat. No. GTX24819

Reference 30

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Xenopus
Package
50 μl ($399)
See all ERK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
IHC-P 1:10-1:100
IHC Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Product Note

The antibody recognizes the same sequence in both proteins (ERK1/2) as long as the T and the Y are phosphorylated. Does not recognize either monophospho- forms of ERK1/2 or the non-phosphorylated proteins.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ERK1 and 2 that contains threonine 202/185 and tyrosine 204/187. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380440

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 1 , mitogen-activated protein kinase 3 , ERK , ERK-2 , ERK2 , ERT1 , MAPK2 , P42MAPK , PRKM1 , PRKM2 , p38 , p40 , p41 , p41mapk , p42-MAPK , ERK-1 , ERK1 , ERT2 , HS44KDAP , HUMKER1A , P44ERK1 , P44MAPK , PRKM3 , p44-ERK1 , p44-MAPK

Cellular Localization

Cytoplasm,Nucleus

Background

kinase involved in intracellular signaling; component of Mapk signaling pathway [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819

GTX24819 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:50

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819

GTX24819 WB Image

Orthogonal

WB (peptide competition) analysis of PC12 cells stimulated with 0.5 M sorbitol for 5 minutes (Lane 2-6) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphothreonine-containing peptide (Lane 4), a generic phosphotyrosine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819

GTX24819 IHC-P Image

IHC-P analysis of mouse stomach tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819

GTX24819 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819

GTX24819 WB Image

Orthogonal

WB analysis of NIH3T3 untreated (Lane 1) or treated with PDGF (Lane 2) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Loading: 5 ug

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819

GTX24819 WB Image

Orthogonal

WB analysis of whole cell extracts (30 μg lysate) of MDA-MB-231 with treatment of EGF(100ng/ml for 15mins) (Lane 1), MDA-MB-231 (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4) and HeLa (Lane 5) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Dilution : 1:1000

REFERENCE

Application Reference

Hung YH et al. Am J Cancer Res 2023; 13 (8) : 3417-3432 Pancreatic cancer cell-derived semaphorin 3A promotes neuron recruitment to accelerate tumor growth and dissemination.
Zhang X et al. Chin Herb Med 2022; 14 (4) : 554-562 Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis.
Application : WB
Reactivity : Mouse
Iwashita J et al. Front Pharmacol 2022; 13 : 851374 Type IV collagen reduces MUC5AC secretion in the lungs of ovalbumin-sensitized mice.
Yan Sun et al. Immun Inflamm Dis 2022; 10 (7) : e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
Application : WB
Reactivity : Human
Jun Iwashita et al. Biochem Biophys Rep 2021; 28 : 101124 Integrin β1 subunit regulates cellular and secreted MUC5AC and MUC5B production in NCI-H292 human lung epithelial cells.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Hung YH et al. Am J Cancer Res 2023; 13 (8):3417-3432 Pancreatic cancer cell-derived semaphorin 3A promotes neuron recruitment to accelerate tumor growth and dissemination.
Zhang X et al. Chin Herb Med 2022; 14 (4):554-562 Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis.
Application : WB
Reactivity : Mouse
Iwashita J et al. Front Pharmacol 2022; 13:851374 Type IV collagen reduces MUC5AC secretion in the lungs of ovalbumin-sensitized mice.
Yan Sun et al. Immun Inflamm Dis 2022; 10 (7):e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
Application : WB
Reactivity : Human
Jun Iwashita et al. Biochem Biophys Rep 2021; 28:101124 Integrin β1 subunit regulates cellular and secreted MUC5AC and MUC5B production in NCI-H292 human lung epithelial cells.
Application : WB
Reactivity : Human
Shih TL et al. J Cell Mol Med 2021; 25 (19):9434-9446 A novel naphthalimide derivative reduces platelet activation and thrombus formation via suppressing GPVI.
Application : WB
Reactivity : Mouse
Li Y et al. Transl Lung Cancer Res 2021; 10 (4):1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.
Application : WB
Reactivity : Human
Huang C et al. BMC Complement Med Ther 2020; 20 (1):68 Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and -independent apoptosis via Smad or Akt signaling pathways in HOS cells.
Navarrete-Bernal MGC et al. Front Oncol 2020; 10:1206 Biological Landscape of Triple Negative Breast Cancers Expressing CTLA-4.
Application : WB
Reactivity : Human
Wang CH et al. Sci Rep 2020; 10 (1):2187 The novel application of cordycepin in maintaining stem cell pluripotency and increasing iPS cell generation efficiency.
Application : WB
Reactivity : Mouse
Yang SS et al. Bone 2019; 130:115073 Tauroursodeoxycholic acid induces angiogenic activity in endothelial cells and accelerates bone regeneration.
Application : WB
Reactivity : Human
Chen YR et al. Food Chem Toxicol 2019; 132:110693 Thyroid hormone-induced expression of inflammatory cytokines interfere with resveratrol-induced anti-proliferation of oral cancer cells.
Application : WB
Reactivity : Human
Huang HY et al. Fish Shellfish Immunol 2019; 90:338-348 Dietary N-Carbamylglutamate (NCG) alleviates liver metabolic disease and hepatocyte apoptosis by suppressing ERK1/2-mTOR-S6K1 signal pathway via promoting endogenous arginine synthesis in Japanese seabass (Lateolabrax japonicus).
Application : WB
Jau?Tien Lin et al. Journal of Food Biochemistry 2019; (Epub) Litchi (Litchi chinensis Sonn.) flower proanthocyanidin fraction exhibited protective efficacy to suppress nickel?induced expression for vascular endothelial growth factor in HepG2 cells.
Application : WB
Reactivity : Human
Hsieh WY et al. PLoS One 2019; 14 (1):e0210656 Renal chymase-dependent pathway for angiotensin II formation mediated acute kidney injury in a mouse model of aristolochic acid I-induced acute nephropathy.
Application : WB
Reactivity : Mouse
Liu W et al. J Anesth 2018; (Epub) effects of hypothermia during * anesthesia on learning and memory ability and hippocampal apoptosis in neonatal rats.
Application : WB
Reactivity : Rat
Huang KH et al. J Endod 2018; 44 (11):1677-1685 Enhanced Capability of Bone Morphogenetic Protein 2-loaded Mesoporous Calcium Silicate Scaffolds to Induce Odontogenic Differentiation of Human Dental Pulp Cells.
Application : WB
Reactivity : Human
Chen CF et al. Oncology Letters 2018;:6323-6330 synergistic inhibitory effects of * and curcumin on human *-resistant oral cancer car cells through intrinsic apoptotic process.
Application : WB
Reactivity : Human
Li JM et al. Ther Adv Med Oncol 2018; 10:1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
Application : WB
Reactivity : Human
Lin JT et al. Journal of Functional Foods 2018; 47:343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Lin C.I. et al. Int. J. Biol. Sci. 2018; 14 (3):253-265 Instillation of particulate matter 2.5 induced acute lung injury and attenuated the injury recovery in ACE2 knockout mice.
Application : WB
Reactivity : Mouse
Yeh CJ et al. Sci Rep 2017; 7 (1):15599 The effects of artocarpin on wound healing: in vitro and in vivo studies.
Application : WB
Reactivity : Human
Cai H et al. J Nat Med 2017; (Epub) 18β-glycyrrhetinic acid inhibits migration and invasion of human gastric cancer cells via the ROS/PKC-α/ERK pathway.
Application : WB
Reactivity : Human
Lin WC et al. RSC Adv. 2017; 7:21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.
Application : WB
Reactivity : Mouse
Chen CC et al. PLoS One 2016; 11 (7):e0158662 The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages.
Application : WB
Reactivity : Mouse
Chen WL et al. PLoS One 2015; 10 (9):e0137979 Mycobacterium tuberculosis Upregulates TNF-α Expression via TLR2/ERK Signaling and Induces MMP-1 and MMP-9 Production in Human Pleural Mesothelial Cells.
Application : WB
Reactivity : Human
Vazquez-Mellado MJ et al. Life Sci 2015; 137 (Epub):142-9 erythropoietin protects neuroblastoma cells against * and * by activating erk and akt pathways but has no effect in kidney cells.
Application : WB
Reactivity : Human
Clone Reference
Robinson FL et al. J Biol Chem 2002; 277 (17):14844-52 Identification of novel point mutations in ERK2 that selectively disrupt binding to MEK1.
Tong T et al. Exp Cell Res 2001; 269 (1):64-72 Involvement of the MAP kinase pathways in induction of GADD45 following UV radiation.
Janulis M et al. Mol Cell Biol 2001; 21 (6):2235-47 A novel mitogen-activated protein kinase is responsive to Raf and mediates growth factor specificity.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399