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Ebola virus VP40 antibody

Anti-Ebola virus VP40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134034
Anti-Ebola virus VP40 antibody used in Western Blot (WB). GTX134034

Cat. No. GTX134034

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Ebola virus
Package
100 μl ($399),
25 μl ($169)
See all Ebola virus VP40 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

Zaire ebolavirus VP40-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Ebola virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Ebola virus VP40 Protein (Zaire ebolavirus (strain Mayinga-76)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887183

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ebola virus Capsid protein VP40 , Ebola virus VP40 , EBOV Capsid protein VP40 , EBOV VP40

Research Area

DATA IMAGES

Anti-Ebola virus VP40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134034

GTX134034 ICC/IF Image

Ebola virus VP40 antibody detects Ebola virus VP40 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ebola virus VP40 protein stained by Ebola virus VP40 antibody (GTX134034) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Ebola virus VP40 antibody used in Western Blot (WB). GTX134034

GTX134034 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ebola virus VP40 antibody (GTX134034) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ermela Paparisto et al. Cells 2021; 10 (9) Interferon-Induced HERC5 Inhibits Ebola Virus Particle Production and Is Antagonized by Ebola Glycoprotein.
Application : WB
Ning Y et al. J Cancer 2021; 12 (22) : 6685-6694 Overexpression of P4HA1 associates with poor prognosis and promotes cell proliferation and metastasis of lung adenocarcinoma.
Application : WB
Pham-Tue-Hung Tran et al. Microorganisms 2020; 8 (12) : E1890 Development of a Multivalent Kunjin Virus Reporter Virus-Like Particle System Inducing Seroconversion for Ebola and West Nile Virus Proteins in Mice.
Application : WB, ICC/IF
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169