Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
1/50-1/100 |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Note :
IHC-P
Clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0, 10mins is recommended for tissues fixed for more than 24 hours.
FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes murine F4/80 antigen which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Unconjugated
RRID
AB_385952
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
adhesion G protein-coupled receptor E1 , DD7A5-7 , EGF-TM7 , Emr1 , F4/80 , Gpf480 , Ly71 , TM7LN3
Cellular Localization
Cell membrane
Database
Research Area