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Flavivirus group antibody [D1-4G2-4-15 (4G2)]

Anti-Flavivirus group antibody [D1-4G2-4-15 (4G2)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57154
Anti-Flavivirus group antibody [D1-4G2-4-15 (4G2)] used in ELISA (ELISA). GTX57154

Cat. No. GTX57154

Reference 14

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 D1-4G2-4-15 (4G2)

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA

Reactivity

 Flavivirus
Package
100 μg ($399)
See all Flavivirus group products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
ELISA Assay dependent

Note :

WB
Binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions.(Lai et al. 2008)
Not tested in other applications.

Product Note

Recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus

Immunogen

Dengue Virus type 2 antigens.

Purification

Protein A affinity purified

Conjugation

Unconjugated

RRID

AB_2887950

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Flavivirus group

Research Area

DATA IMAGES

Anti-Flavivirus group antibody [D1-4G2-4-15 (4G2)] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57154

GTX57154 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed Vero cells infected with ZIKV after 30h infection using GTX57154 Flavivirus group antibody [D1-4G2-4-15 (4G2)]. Staining was performed in 0.3% Triton X-100 at 1:20 dilution overnight at 4ºC.
Red : Primary antibody
Blue : DAPI

Anti-Flavivirus group antibody [D1-4G2-4-15 (4G2)] used in ELISA (ELISA). GTX57154

GTX57154 ELISA Image

ELISA analysis of Dengue Virus Serotype 2 VLPs (5μg/ml) using serial diluted GTX57154 Flavivirus group antibody [D1-4G2-4-15 (4G2)].

REFERENCE

Application Reference

HL Dong et al. Vaccines (Basel) 2024; 12 (2) : 215 The Chimeric Chaoyang-Zika Vaccine Candidate Is Safe and Protective in Mice
Application : ICC/IF
Dong HL et al. Vaccines (Basel) 2023; 11 (7) Rapid Generation of Recombinant Flaviviruses Using Circular Polymerase Extension Reaction.
Yang L et al. iScience 2023; 26 (7) : 107001 Isogenic human trophectoderm cells demonstrate the role of NDUFA4 and associated variants in ZIKV infection.
Hao-Long Dong et al. Vaccines 2023; 11 (7) : 1250 Rapid Generation of Recombinant Flaviviruses Using Circular Polymerase Extension Reaction
Application : ICC/IF
Garcia G Jr et al. Cell Rep Med 2023; 4 (5) : 101024 Innate immune pathway modulator screen identifies STING pathway activation as a strategy to inhibit multiple families of arbo and respiratory viruses.
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REVIEW

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Application Reference
HL Dong et al. Vaccines (Basel) 2024; 12 (2):215 The Chimeric Chaoyang-Zika Vaccine Candidate Is Safe and Protective in Mice
Application : ICC/IF
Dong HL et al. Vaccines (Basel) 2023; 11 (7) Rapid Generation of Recombinant Flaviviruses Using Circular Polymerase Extension Reaction.
Yang L et al. iScience 2023; 26 (7):107001 Isogenic human trophectoderm cells demonstrate the role of NDUFA4 and associated variants in ZIKV infection.
Hao-Long Dong et al. Vaccines 2023; 11 (7):1250 Rapid Generation of Recombinant Flaviviruses Using Circular Polymerase Extension Reaction
Application : ICC/IF
Garcia G Jr et al. Cell Rep Med 2023; 4 (5):101024 Innate immune pathway modulator screen identifies STING pathway activation as a strategy to inhibit multiple families of arbo and respiratory viruses.
Wang HW et al. J Biomed Sci 2023; 30 (1):12 CLEC5A mediates Zika virus-induced testicular damage.
Han Y et al. Cell Stem Cell 2022; 29 (10):1475-1490.e6 A human iPSC-array-based GWAS identifies a virus susceptibility locus in the NDUFA4 gene and functional variants.
Application : ICC/IF
Bagchi P et al. PLoS Pathog 2022; 18 (7):e1010717 A specific EMC subunit supports Dengue virus infection by promoting virus membrane fusion essential for cytosolic genome delivery.
Sannigrahi A et al. Membranes (Basel) 2022; 12 (12) A Versatile Suspended Lipid Membrane System for Probing Membrane Remodeling and Disruption.
Achinta Sannigrahi et al. Membranes 2022; 12 A Versatile Suspended Lipid Membrane System for Probing Membrane Remodeling and Disruption
Application : ICC/IF
Remmel JL et al. Virology 2021; 559:57-64 Diverse contributions of avidity to the broad neutralization of Dengue virus by antibodies targeting the E dimer epitope.
Ma L et al. EMBO Rep 2021; 22 (8):e51978 Reversing neural circuit and behavior deficit in mice exposed to maternal inflammation by Zika?virus.
Application : IHC-Fr
Luisi K et al. Sci Adv 2020; 6 (32):eaba5068 Development of a potent Zika virus vaccine using self-amplifying messenger RNA.
Application : WB
Clone Reference
Lai CY et al. J Virol 2008; 82 (13):6631-43 Antibodies to envelope glycoprotein of dengue virus during the natural course of infection are predominantly cross-reactive and recognize epitopes containing highly conserved residues at the fusion loop of domain II.
Application : WB
SDS
PBS.pdf
Proclin.pdf
Package List Price ($)
$ 399