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Fluoroshield™ with DAPI

Fluoroshield™ with DAPI. GTX30920

Cat. No. GTX30920

Reference 41

Application

 ICC/IF, IHC-Fr, IHC
Package
20 ml ($149)
See all Mounting Medium products
PRODUCT

Summary

Fluoroshield™ with DAPI

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Storage

Store at 2-8ºC, Protect from light, DO NOT FREEZE.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Fluoroshield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI.

DATA IMAGES

Fluoroshield™ with DAPI. GTX30920

GTX30920 ICC/IF Image

Fluoroshield™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This mounting medium is fortified with DAPI which is a counterstain for DNA.

REFERENCE

Application Reference

YC Wang et al. Mater Adv 2024; Europium-doped hybrid nano-complexes: a potential strategy for metastasis prevention in osteosarcoma
Liu Y et al. Cell Commun Signal 2024; 22 (1) : 155 Extracellular vesicles from UTX-knockout endothelial cells boost neural stem cell differentiation in spinal cord injury.
Xue B et al. Sci Rep 2024; 14 (1) : 837 Circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of diabetic cystopathy.
Theranostics 2024; 14 (2) : 510-527 Exosomes derived from CD271+CD56+ bone marrow mesenchymal stem cell subpopoulation identified by single-cell RNA sequencing promote axon regeneration after spinal cord injury
T Qin et al. Bioact Mater 2023; 33 : 424-443 Local delivery of EGFR+NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway
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REVIEW

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Application Reference
YC Wang et al. Mater Adv 2024; Europium-doped hybrid nano-complexes: a potential strategy for metastasis prevention in osteosarcoma
Liu Y et al. Cell Commun Signal 2024; 22 (1):155 Extracellular vesicles from UTX-knockout endothelial cells boost neural stem cell differentiation in spinal cord injury.
Xue B et al. Sci Rep 2024; 14 (1):837 Circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of diabetic cystopathy.
Theranostics 2024; 14 (2):510-527 Exosomes derived from CD271+CD56+ bone marrow mesenchymal stem cell subpopoulation identified by single-cell RNA sequencing promote axon regeneration after spinal cord injury
T Qin et al. Bioact Mater 2023; 33:424-443 Local delivery of EGFR+NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway
FF Chen et al. Kaohsiung J Med Sci 2023; SIRT3 enhances the protective effect of Xyloketal B on seizure-induced brain injury by regulating AMPK/mTOR signaling-mediated autophagy
AK Pfitzner et al. J Cell Biol 2023; Vps60 initiates alternative ESCRT-III filaments
W Peng et al. J Nanobiotechnology 2023; UTX deletion promotes M2 macrophage polarization by epigenetically regulating endothelial cell-macrophage crosstalk after spinal cord injury
He N et al. Nat Commun 2023; 14 (1):3063 Photoinhibiting via simultaneous photoabsorption and free-radical reaction for high-fidelity light-based bioprinting.
Su CL et al. Commun Biol 2022; 5 (1):1166 Wireless neuromodulation in vitro and in vivo by intrinsic TRPC-mediated magnetomechanical stimulation.
Anshu Agarwal et al. Microbiol Spectr 2022; 10 (3):e0083022 Japanese Encephalitis Virus NS4A Protein Interacts with PTEN-Induced Kinase 1 (PINK1) and Promotes Mitophagy in Infected Cells.
Jianming Wang et al. Cell Rep 2021; 34 (7):108759 Persistence of RNA transcription during DNA replication delays duplication of transcription start sites until G2/M.
Yu-En Lin et al. Elife 2021; 10 Glial Nrf2 signaling mediates the neuroprotection exerted by Gastrodia elata Blume in Lrrk2-G2019S Parkinson's disease.
Osburn SC et al. Physiologia 2021; 1 (1):22-33 Proteasome- and Calpain-Mediated Proteolysis, but Not Autophagy, Is Required for Leucine-Induced Protein Synthesis in C2C12 Myotubes.
Yen YT et al. Nat Commun 2021; 12 (1):7297 Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response.
Osburn S.C. et al. Physiologia 2021; 1 (1):22-33 Proteasome- and Calpain-Mediated Proteolysis, but Not Autophagy, Is Required for Leucine-Induced Protein Synthesis in C2C12 Myotubes.
Salih S et al. Exp Neurol 2021; 348:113950 Sox-positive cell population in the adult cerebellum increases upon tissue degeneration.
Peng W et al. Oxid Med Cell Longev 2021; 2021:1695087 Microglia-Derived Exosomes Improve Spinal Cord Functional Recovery after Injury via Inhibiting Oxidative Stress and Promoting the Survival and Function of Endothelia Cells.
Joviano-Santos JV et al. FEBS J 2021; Motoneuron-specific loss of VAChT mimics neuromuscular defects seen in congenital myasthenic syndrome.
Wu CY et al. Theranostics 2020; 10 (8):3546-3561 Targeted delivery of engineered auditory sensing protein for ultrasound neuromodulation in the brain.
Wilson E et al. J Neurosci Res 2020; (Epub) Cytoskeletal regulation of synaptogenesis in a model of human fetal brain development.
Ahmed E et al. J Biomed Mater Res A 2020; (Epub) Decellularized extracellular matrix-rich hydrogel-silver nanoparticle mixture as a potential treatment for acute liver failure model.
Roberts MD et al. Am J Physiol Regul Integr Comp Physiol 2019; (Epub) Synergist ablation-induced hypertrophy occurs more rapidly in the plantaris than soleus muscle in rats due to different molecular mechanisms.
Kumar Singh A et al. Mol Immunol 2019; 116:63-72 Splicing regulator SRSF1-3 that controls somatic hypermutation of IgV genes interacts with topoisomerase 1 and AID.
Shahror RA et al. J Neurotrauma 2019; (Epub) Transplantation of Mesenchymal Stem Cells Overexpressing FGF21 Facilitates Cognitive Recovery and Enhances Neurogenesis in a Mouse Model of Traumatic Brain Injury.
Shahror RA et al. Int J Mol Sci 2019; 20 (11) Enhanced Homing of Mesenchymal Stem Cells Overexpressing Fibroblast Growth Factor 21 to Injury Site in a Mouse Model of Traumatic Brain Injury.
Jacobsen M et al. Front Neurol 2018; 9:1107 Activation of the Large-Conductance, Voltage, and Ca2+- Activated K+ (BK) Channel in Acute Spinal Cord Injury in the Wistar Rat Is Neuroprotective.
Chuang HC et al. Sci Adv 2018; 4 (9):eaat5401 GLK-IKKβ signaling induces dimerization and translocation of the AhR-RORγt complex in IL-17A induction and autoimmune disease.
Chih-Chien Sung et al. bioRxiv 2018; (Epub) RNA-Seq and Protein Mass Spectrometry in Microdissected Kidney Tubules Reveal Signaling Processes that Initiate Lithium-Induced Diabetes Insipidus.
Kimura S et al. J Atheroscler Thromb 2018; (Epub) Relationship between CCL22 Expression by Vascular Smooth Muscle Cells and Macrophage Histamine Receptors in Atherosclerosis.
Taschner M et al. Elife 2018; 7 Crystal structure of intraflagellar transport protein 80 reveals a homo-dimer required for ciliogenesis.
Sharma Y et al. Gene Expr 2018; (Epub) In Atp7b-/- Mice Modeling Wilson's Disease Liver Repopulation with Bone Marrowderived Myofibroblasts or Inflammatory Cells and not Hepatocytes is Deleterious.
Lin CH et al. Exp Cell Res 2017; 359 (2):367-373 Activation of AMPK is neuroprotective in the oxidative stress by advanced glycosylation end products in human neural stem cells.
Wu PK et al. Stem Cells Transl Med 2017; 6 (6):1504-1514 Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity.
Rodriguez-Lara V et al. Endocr Res 2017;:43113 Estrogen induces CXCR4 overexpression and CXCR4/CXL12 pathway activation in lung adenocarcinoma cells in vitro.
Saxena A et al. Cancer Biol Ther 2017;:0 Dietary selenium protects adiponectin knockout mice against chronic inflammation induced colon cancer.
Fan CH et al. Biomaterials 2016; 106:46-57 Folate-conjugated gene-carrying microbubbles with focused ultrasound for concurrent blood-brain barrier opening and local gene delivery.
Duerrschmid C et al. Circ Heart Fail 2015; 8 (2):352-61 Tumor necrosis factor: a mechanistic link between angiotensin-II-Induced cardiac inflammation and fibrosis.
Kowalewska PM et al. PLoS One 2014; 9 (9):e104537 Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
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Fan CH et al. Biomaterials 2013; 34 (8):2142-55 Antiangiogenic-targeting drug-loaded microbubbles combined with focused ultrasound for glioma treatment.
Package List Price ($)
$ 149