APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain , 293T , HMGB1-transfected 293T
Predict Reactivity
Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888111
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1
Cellular Localization
Nucleus
Background
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
Database
Research Area
DATA IMAGES
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/templates/default/js/lightbox/images/loading.gif "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
|
GTX629445 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:2000.
|
|
|
GTX629445 ICC/IF Image
HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:500.
Red: GFAP, a glia cell marker, stained by GFAP antibody (GTX27260) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
|
|
|
GTX629445 IHC-Fr Image
HMGB1 antibody [GT427] detects HMGB1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: HMGB1 stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:250.
Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 5 min
|
|
|
GTX629445 WB Image
HMGB1 antibody [GT427] detects HMGB1 protein by western blot analysis. Mouse tissue extracts and Rat tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:1000.
|
|
|
GTX629445 ICC/IF Image
HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.
|
|
|
GTX629445 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:3000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
|
GTX629445 WB Image
HMGB1 antibody [GT427] detects 0 protein by Western blot analysis.
A. 30 μg SK-N-SH whole cell lysate/extract
B. 30 μg IMR32 whole cell lysate/extract
C. 30 μg SK-N-AS whole cell lysate/extract
12 % SDS-PAGE
HMGB1 antibody [GT427] (GTX629445) dilution: 1:1000
|
|
|
GTX629445 ICC/IF Image
HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody (GTX133109) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
|
REFERENCE
REVIEW
There are currently no reviews for HMGB1 antibody [GT427] (GTX629445). Be the first to share your experience with this product.
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20160602_WB_shRNA_watermark_w_23061202_514.webp "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20170727_IFA_R_w_23061202_252.webp "Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445")
![Anti-HMGB1 antibody [GT427] used in IHC (Frozen sections) (IHC-Fr). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20170829_IHC-Fr_M_w_23061202_608.webp "Anti-HMGB1 antibody [GT427] used in IHC (Frozen sections) (IHC-Fr). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20151015_WB_Merged_w_23061202_573.webp "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20160824_IFA_w_23061202_355.webp "Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20181005_WB_B_w_23061202_295.webp "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_WB_w_23061202_393.webp "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445](/upload/website/prouct_img/small/GTX629445/GTX629445_41344_20170705_IFA_R_w_23061202_153.webp "Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20160602_WB_shRNA_watermark_w_23061202_514.webp "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20170727_IFA_R_w_23061202_252.webp "Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445")
![Anti-HMGB1 antibody [GT427] used in IHC (Frozen sections) (IHC-Fr). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20170829_IHC-Fr_M_w_23061202_608.webp "Anti-HMGB1 antibody [GT427] used in IHC (Frozen sections) (IHC-Fr). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20151015_WB_Merged_w_23061202_573.webp "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20160824_IFA_w_23061202_355.webp "Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20181005_WB_B_w_23061202_295.webp "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_WB_w_23061202_393.webp "Anti-HMGB1 antibody [GT427] used in Western Blot (WB). GTX629445")
![Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445](/upload/website/prouct_img/normal/GTX629445/GTX629445_41344_20170705_IFA_R_w_23061202_153.webp "Anti-HMGB1 antibody [GT427] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629445")