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IRF3 antibody [4D4]

Overexpression
Anti-IRF3 antibody [4D4] used in Flow cytometry (FACS). GTX84288
Anti-IRF3 antibody [4D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84288
Anti-IRF3 antibody [4D4] used in Western Blot (WB). GTX84288
Anti-IRF3 antibody [4D4] used in IHC (Paraffin sections) (IHC-P). GTX84288
Anti-IRF3 antibody [4D4] used in Western Blot (WB). GTX84288

Cat. No. GTX84288

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4D4

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all IRF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human IRF3 (NP_001562) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 3 , IIAE7

Cellular Localization

Cytoplasm,Nucleus

Background

Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.

Database

Research Area

DATA IMAGES

Anti-IRF3 antibody [4D4] used in Flow cytometry (FACS). GTX84288

GTX84288 FACS Image

FACS analysis of HEK293T cells transfected with either IRF3 plasmid(Red) or empty vector control plasmid(Blue) using GTX84288 IRF3 antibody [4D4].

Anti-IRF3 antibody [4D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84288

GTX84288 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with IRF3 plasmid using GTX84288 IRF3 antibody [4D4].

Anti-IRF3 antibody [4D4] used in Western Blot (WB). GTX84288

GTX84288 WB Image

WB analysis of various cell lines using GTX84288 IRF3 antibody [4D4].
Loading : 35 ug per lane

Anti-IRF3 antibody [4D4] used in IHC (Paraffin sections) (IHC-P). GTX84288

GTX84288 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84288 IRF3 antibody [4D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IRF3 antibody [4D4] used in Western Blot (WB). GTX84288

GTX84288 WB Image

Overexpression

WB analysis of HEK293T cells transfected with IRF3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84288 IRF3 antibody [4D4].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519