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MEK1 antibody

Comparison
Anti-MEK1 antibody used in Western Blot (WB). GTX102391
Anti-MEK1 antibody used in Western Blot (WB). GTX102391
Anti-MEK1 antibody used in Western Blot (WB). GTX102391
Anti-MEK1 antibody used in Western Blot (WB). GTX102391
Anti-MEK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102391
Anti-MEK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102391

Cat. No. GTX102391

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all MEK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MEK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950857

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 1 , CFC3 , MAPKK1 , MEK1 , MKK1 , PRKMK1

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MEK1 antibody used in Western Blot (WB). GTX102391

GTX102391 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 antibody (GTX102391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MEK1 antibody used in Western Blot (WB). GTX102391

GTX102391 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX102391 diluted at 1:1000

Anti-MEK1 antibody used in Western Blot (WB). GTX102391

GTX102391 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX102391 diluted at 1:3000

Anti-MEK1 antibody used in Western Blot (WB). GTX102391

GTX102391 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with MEK1 antibody (GTX102391) diluted at 1:1000 and competitor's antibody (SC-219) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-MEK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102391

GTX102391 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using MEK1(GTX102391) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-MEK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102391

GTX102391 IHC-P Image

MEK1 antibody detects MEK1 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
MEK1 antibody (GTX102391) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Jun Hyoung Jeon et al. Neurotherapeutics 2021; 18 (3) : 1768-1782 The Antipsychotic Drug Clozapine Suppresses the RGS4 Polyubiquitylation and Proteasomal Degradation Mediated by the Arg/N-Degron Pathway.
Application : WB
Reactivity : Human
Tang WF et al. J Proteome Res 2018; (Epub) Large-Scale Proteomic Identification of Targets of Cellular miR-197 Downregulated by Enterovirus A71.
Application : WB
Reactivity : Human
Wang TH et al. Int J Mol Sci 2018; 19 (9) Melatonin Inhibits the Progression of Hepatocellular Carcinoma through MicroRNA Let7i-3p Mediated RAF1 Reduction.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169