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Mouse IgG isotype control

Cat. No. GTX35009

Reference 20

Host

 Mouse

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, ELISA, ChIP assay, Blocking, in vitro, in vivo
Package
100 μg ($119)
See all Mouse IgG isotype control products

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

PROPERTIES

Form

Liquid

Buffer

Borate buffered saline

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Isotype control for Mouse IgG , Polyclonal Mouse IgG isotype control , Mouse IgG negative control , Mouse IgG isotype control , Negative control for Mouse IgG , Mouse isotype control for IgG , Isotype control to Mouse IgG , Mouse negative control for IgG

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

Application Reference

WF Wei et al. J Exp Clin Cancer Res 2023; 42 (1) : 160 Cancer-associated fibroblast-derived PAI-1 promotes lymphatic metastasis via the induction of EndoMT in lymphatic endothelial cells
Barreto-Duran E et al. Front Immunol 2022; 13 : 991991 The interplay between the airway epithelium and tissue macrophages during the SARS-CoV-2 infection.
Zhang X et al. Acta Biochim Biophys Sin (Shanghai) 2022; 54 (10) : 1518-1527 Identification and characterization of blocking nanobodies against human CD70.
Hsin-I Wang et al. Int J Mol Sci 2021; 22 (16) Small Structural Proteins E and M Render the SARS-CoV-2 Pseudovirus More Infectious and Reveal the Phenotype of Natural Viral Variants.
Simko EAJ et al. Nucleic Acids Res 2020; 48 (13) : 7421-7438 G-quadruplexes offer a conserved structural motif for NONO recruitment to NEAT1 architectural lncRNA.
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REVIEW

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Application Reference
WF Wei et al. J Exp Clin Cancer Res 2023; 42 (1):160 Cancer-associated fibroblast-derived PAI-1 promotes lymphatic metastasis via the induction of EndoMT in lymphatic endothelial cells
Barreto-Duran E et al. Front Immunol 2022; 13:991991 The interplay between the airway epithelium and tissue macrophages during the SARS-CoV-2 infection.
Zhang X et al. Acta Biochim Biophys Sin (Shanghai) 2022; 54 (10):1518-1527 Identification and characterization of blocking nanobodies against human CD70.
Hsin-I Wang et al. Int J Mol Sci 2021; 22 (16) Small Structural Proteins E and M Render the SARS-CoV-2 Pseudovirus More Infectious and Reveal the Phenotype of Natural Viral Variants.
Simko EAJ et al. Nucleic Acids Res 2020; 48 (13):7421-7438 G-quadruplexes offer a conserved structural motif for NONO recruitment to NEAT1 architectural lncRNA.
Hamasaki M et al. Sci Rep 2020; 10 (1):7558 Transcriptional profiling of murine macrophages stimulated with cartilage fragments revealed a strategy for treatment of progressive osteoarthritis.
Lin MW et al. Antioxidants (Basel) 2019; 9 (1) Celastrol Inhibits Dopaminergic Neuronal Death of Parkinson's Disease through Activating Mitophagy.
Li C et al. Front Oncol 2019; 9:225 BAG3 Suppresses Loading of Ago2 to IL6 mRNA in Pancreatic Ductal Adenocarcinoma.
Chang L et al. Nucleic Acids Res 2019; 47 (2):824-842 ITCH nuclear translocation and H1.2 polyubiquitination negatively regulate the DNA damage response.
Hagenau F et al. Am J Ophthalmol 2018; (Epub) Vitrectomy for diabetic macular edema: optical coherence tomography criteria and pathology of the vitreomacular interface.
Li M et al. PLoS One 2018; 13 (7):e0200478 Dengue immune sera enhance Zika virus infection in human peripheral blood monocytes through Fc gamma receptors.
Liu D et al. Nat Commun 2018; 9 (1):2612 Peptide density targets and impedes triple negative breast cancer metastasis.
Huang YJ et al. Nanotheranostics 2017; 1 (3):326-337 TRAIL-functionalized gold nanoparticles selectively trigger apoptosis in polarized macrophages.
Shih JW et al. Nat Commun 2017; 8:15874 Long noncoding RNA LncHIFCAR/MIR31HG is a HIF-1α co-activator driving oral cancer progression.
Yin JJ et al. Chin Med J (Engl) 2017; 130 (8):964-971 Neutralization of Interleukin-9 Decreasing Mast Cells Infiltration in Experimental Autoimmune Encephalomyelitis.
Yang CC et al. PLoS One 2014; 9 (10):e106683 Nuclear export of human hepatitis B virus core protein and pregenomic RNA depends on the cellular NXF1-p15 machinery.
Lane JS et al. Langmuir 2014; 30 (31):9457-65 Rational targeting of subclasses of intermolecular interactions: elimination of nonspecific binding for analyte sensing.
Wang HJ et al. Proc Natl Acad Sci U S A 2014; 111 (1):279-84 JMJD5 regulates PKM2 nuclear translocation and reprograms HIF-1α-mediated glucose metabolism.
Schittenhelm J et al. Int J Clin Exp Pathol 2013; 6 (12):2719-32 Comparing the expression of integrins αvβ3, αvβ5, αvβ6, αvβ8, fibronectin and fibrinogen in human brain metastases and their corresponding primary tumors.
Griffin CA et al. J Cell Sci 2010; 123 (Pt 18):3052-60 Chemokine expression and control of muscle cell migration during myogenesis.
Package List Price ($)
$ 119