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Rat IgG isotype control

Cat. No. GTX35047

Reference 2

Host

 Rat

Clonality

 Polyclonal

Isotype

 IgG

Application

 FACS, ELISA
Package
100 μg ($99)
See all Rat IgG isotype control products

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

PROPERTIES

Form

Liquid

Buffer

Borate buffered saline

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Isotype control to Rat IgG , Rat negative control for IgG , Polyclonal Rat IgG isotype control , Rat IgG isotype control , Isotype control for Rat IgG , Rat IgG negative control , Rat isotype control for IgG , Negative control for Rat IgG

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

Application Reference

Namangkalakul W et al. J Tissue Eng 2023; 14 : 20417314231187960 Augmented effect of fibroblast growth factor 18 in bone morphogenetic protein 2-induced calvarial bone healing by activation of CCL2/CCR2 axis on M2 macrophage polarization.
Zheng F et al. BioMed Research International 2017; 2017 : 1293201 PD-L1 Promotes Self-Renewal and Tumorigenicity of Malignant Melanoma Initiating Cells.
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Package List Price ($)
$ 99