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Rat IgG1 isotype control [KLH/G1-2-2] (Biotin)

Cat. No. GTX35053

Reference 1

Host

 Rat

Clonality

 Monoclonal

Clone Name

 KLH/G1-2-2

Isotype

 IgG1

Application

 FACS, ELISA
Package
100 μg ($179)
See all Rat IgG1 isotype control products

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

Product Note

Keyhole limpet hemocyanin (KLH)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Keyhole limpet hemocyanin (KLH)

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

monoclonal Rat IgG1 isotype control , Rat IgG1 kappa isotype control , Rat isotype , Rat IgG1 kappa negative control , Isotype control to Rat IgG1 kappa , Isotype control for Rat IgG1 kappa , Rat IgG1 negative control , Rat negative control for IgG1 kappa

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

Application Reference

Sudworth A et al. J Leukoc Biol 2017; (Epub) Frontline Science: A hyporesponsive subset of rat NK cells negative for Ly49s3 and NKR-P1B are precursors to the functionally mature NKR-P1B+ subset.
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SDS
PBS.pdf
Package List Price ($)
$ 179