SOX2 antibody [GT1876]

Name: SOX2 antibody [GT1876]
Brand: GeneTex
SKU: GTX627404
Price: 399.00 USD
Rating: 4 (2 reviews)

Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

Anti-SOX2 antibody [GT1876] used in Immunoprecipitation (IP). GTX627404

Anti-SOX2 antibody [GT1876] used in Flow cytometry (FACS). GTX627404

Anti-SOX2 antibody [GT1876] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627404

Cat. No. GTX627404

Reference 14

Host	Mouse
Clonality	Monoclonal
Clone Name	GT1876
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
Reactivity	Human, Mouse, Rat, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all SOX2 products

View product citations for antibody GTX627404 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	Assay dependent
IHC-P	Assay dependent
IHC-Fr	1:100-1:1000
FACS	1:50-1:200
IP	1:100-1:500
Note : IHC-Fr The use of GTX627404 for zebrafish is based on WB testing. For IHC or IHC whole mount using zebrafish samples, we recommend GTX124477.

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

human ESC , zebrafish eye , NT2D1 , PC-3 , U87-MG , SK-N-SH

Predict Reactivity

Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_11162865

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY-box 2 , ANOP3 , MCOPS3

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

Database

Gene ID: 6657 SOX2
UniProt: P48431 SOX2

Research Area

DATA IMAGES

GTX627404 IHC-Fr Image

SOX2 antibody [GT1876] detects SOX2 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Red: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX627404 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX627404 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
C: U87-MG
D: SK-N-SH
10% SDS PAGE
GTX627404 diluted at 1:5000

GTX627404 WB Image

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.

GTX627404 IHC-Fr Image

SOX2 antibody [GT1876] detects SOX2 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX627404 WB Image

SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.
A. 30 μg zebrafish eye lysate/extract
10 % SDS-PAGE
SOX2 antibody [GT1876] (GTX627404) dilution: 1:500

GTX627404 WB Image

SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10 % SDS-PAGE
SOX2 antibody [GT1876] (GTX627404) dilution: 1:5000

GTX627404 IP Image

Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [GT1876] (GTX627404) or SOX2 antibody [N1C3] (GTX101057).
Western blot analysis was performed using SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX627404 IHC-Fr Image

Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX100850) diluted at 1:500.
Red: Sox2 antibody [GT1876] (GTX627404) diluted at 1:500.

GTX627404 FACS Image

SOX2 antibody [GT1876] detects SOX2 protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: SOX2 antibody [GT1876] dilution: 1:50