SOX2 antibody

Name: SOX2 antibody
Brand: GeneTex
SKU: GTX101506
Price: 399.00 USD

Anti-SOX2 antibody used in Western Blot (WB). GTX101506

Anti-SOX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101506

Anti-SOX2 antibody used in Flow cytometry (FACS). GTX101506

Anti-SOX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101506

Cat. No. GTX101506

Reference 9

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr, FACS
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all SOX2 products

View product citations for antibody GTX101506 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-Fr	1:100-1:1000
FACS	1:50-1:200

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Human ESC , GL261 , A431 , NT2D1

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037810

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY-box 2 , ANOP3 , MCOPS3

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

Database

Gene ID: 6657 SOX2
UniProt: P48431 SOX2

Research Area

DATA IMAGES

GTX101506 WB Image

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101506 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody (GTX101506) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX101506 WB Image

Sample (30 μg of whole cell lysate)
A: GL261
10% SDS PAGE
GTX101506 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101506 IHC-Fr Image

Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5). Red: Sox2 antibody (GTX101506) diluted at 1:500.
Blue: DAPI

GTX101506 FACS Image

Flow cytometry on human embryonic stem cells, staining with SOX2 (GTX101506)antibody at 1:50 dilution(green) or rabbit IgG (black).

GTX101506 WB Image

Sample (20 μg of whole cell lysate )
A: A431 (GTX27909)
10% SDS PAGE
GTX101506 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101506 WB Image

Sample (20 μg of whole cell lysate)
A: Human ESC
10% SDS PAGE
GTX101506 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101506 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed human embryonic stem cell, using SOX2(GTX101506) antibody at 1:100 dilution.

GTX101506 IHC-Fr Image

Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5).
Green: beta III Tubulin antibody [GT886] (GTX631830) diluted at 1:2000.
Red: Sox2 antibody (GTX101506) diluted at 1:250.

GTX101506 ICC/IF Image

SOX2 antibody detects SOX2 protein at nucleus by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOX2 protein stained by SOX2 antibody (GTX101506) diluted at 1:200.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.