GeneTex
  • Country / Location Selection

United States (US)

alpha Tubulin antibody [GT114]

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802
Anti-alpha Tubulin antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in IHC (Frozen sections) (IHC-Fr). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802
Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

Cat. No. GTX628802

Host

Mouse

Clonality

Monoclonal

Clone Name

GT114

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

Human, Mouse, Rat, Zebrafish, Goat, Drosophila, Crucian carp
Package
100 μl ($399),
25 μl ($169)
PRODUCT

Summary

alpha Tubulin antibody [GT114] detects alpha Tubulin, a cytoskeletal protein widely expressed in cells that is commonly used as a loading control for western blots. The predicted molecular weight of alpha Tubulin protein is 50 kDa. This is a well-cited mouse monoclonal antibody validated for multiple applications including western blot, immunohistochemistry, and immunocytochemistry.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , mouse brain , rat brain , zebrafish eye , PC-12 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2716636

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , tubulin , alpha , ubiquitous antibody , tubulin alpha-1B chain antibody , tubulin , alpha 1b antibody

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX628802

GTX628802 IHC-P Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX628802) at a dilution of 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802

GTX628802 ICC/IF Image

TUBA1B antibody detects TUBA1B protein at Cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min.
Green: TUBA1B protein stained by TUBA1B antibody (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-alpha Tubulin antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX628802

GTX628802 IHC-P Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802

GTX628802 ICC/IF Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in IHC (Frozen sections) (IHC-Fr). GTX628802

GTX628802 IHC-Fr Image

Immunofluorescence photomicrographs of frozen sections of mouse brain.
Green: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:200. The signal was developed using goat anti-rmouse IgG antibody (Dylight488) (GTX213111-04).
Blue: Nuclear staining with Hoechst 33342.

Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802

GTX628802 ICC/IF Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:250.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg mouse brain lysate/extract
D. 30 μg PC-12 whole cell lysate/extract
E. 30 μg rat brain lysate/extract
F. 30 μg drosophila lysae/extract
10% SDS-PAGE
alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis.
A. 30 μg zebrafish eye lysate/extract
10% SDS-PAGE
alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] used in IHC (Paraffin sections) (IHC-P). GTX628802

GTX628802 IHC-P Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal Nat Commun in 2016.PMID: 27221592

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal Life Sci Alliance in 2019.PMID: 31767615

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal PLoS Biol in 2019.PMID: 30883547

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal Biomolecules in 2020.PMID: 32635653

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal Genome Biol in 2018.PMID: 29548335

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal Biomolecules in 2020.PMID: 32635653

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal PLoS One in 2017.PMID: 28467493

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal Physiol Rep in 2017.PMID: 28408640

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal Nat Commun in 2019.PMID: 31278365

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal PLoS Biol in 2019.PMID: 30883547

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal Genome Biol in 2018.PMID: 29548335

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the 2018 in Sci Rep. PMID: 30459446

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35055077

Anti-alpha Tubulin antibody [GT114] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628802

GTX628802 ICC/IF Image

The data was published in the 2018 in Sci Rep. PMID: 30459446

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35055077

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal PLoS One in 2014. PMID: 25000307

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal Cell Biosci in 2019. PMID: 31044068

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal Toxins (Basel) in 2019. PMID: 31159487

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal Nat Commun in 2020. PMID: 32005813

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal PLoS One in 2015. PMID: 25811469

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal J Cell Mol Med in 2020. PMID: 31821701

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal PLoS One in 2015. PMID: 25811469

Anti-alpha Tubulin antibody [GT114] used in Western Blot (WB). GTX628802

GTX628802 WB Image

The data was published in the journal J Cell Mol Med in 2020. PMID: 31821701

REFERENCE

REVIEW

alpha Tubulin antibody [GT114] Cat. No. GTX628802

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 08-Jun-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : SW480 cells
Amount used : 40 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:20000, 4°C, 16Hr
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : U87-MG cells
Blocking : 5% BSA in PBST, 25°C, 2Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Application Reference
Tsai LH et al. Sci Rep 2020; 10 (1):17817
Application : WB
Reactivity : Human
Clone Reference
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169