beta Actin antibody [GT5512]

Name: beta Actin antibody [GT5512]
Brand: GeneTex
SKU: GTX629630
Price: 399.00 USD
Rating: 4.6 (8 reviews)

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

Anti-beta Actin antibody [GT5512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629630

Anti-beta Actin antibody [GT5512] used in IHC (Paraffin sections) (IHC-P). GTX629630

Cat. No. GTX629630

Reference 151

Host	Mouse
Clonality	Monoclonal
Clone Name	GT5512
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat, Zebrafish, Yeast, Rabbit, Goat, Drosophila, Cat, Dog, Monkey, Xenopus
Package	100 μl ($399), 25 μl ($169)

See all beta Actin products

View product citations for antibody GTX629630 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	Assay dependent

Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

30 hpf zebrafish , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , rat brain , rabbit blood , goat blood , MDA-MB-231

Product Note

IP/MS validation was supported by references (PMID:30377426)

Predict Reactivity

Sheep, Bovine, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2728646

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Gene ID: 60 ACTB
UniProt: P60709 ACTB

Research Area

DATA IMAGES

GTX629630 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [GT5512] (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg 293T whole cell lysate/extract
D. 30 μg A431 whole cell lysate/extract
E. 30 μg HeLa whole cell lysate/extract
F. 30 μg HepG2 whole cell lysate/extract
G. 30 μg H1299 whole cell lysate/extract
H. 30 μg HCT116 whole cell lysate/extract
I. 30 μg MCF-7 whole cell lysate/extract
J. 30 μg NT2D1 whole cell lysate/extract
K. 30 μg PC-3 whole cell lysate/extract
L. 30 μg U87-MG whole cell lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:20000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629630 ICC/IF Image

beta Actin antibody [GT5512] detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: beta Actin protein stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 20 μg 293T whole cell lysate/extract
B. 10 μg 293T whole cell lysate/extract
C. 5 μg 293T whole cell lysate/extract
D. 1 μg 293T whole cell lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg drosophila lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg goat blood lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg rabbit blood lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629630 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629630 IHC-P Image

beta Actin antibody [GT5512] detects beta Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
beta Actin stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629630 WB Image

GTX629630 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: PG-4 cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin stained by beta Actin antibody? (GTX629630) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX629630 WB Image

Various whole cell extracts (15 | 5 | 2 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [GT5512] (GTX629630) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg 30 hpf zebrafish lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629630 WB Image

beta Actin antibody[GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
10% SDS-PAGE
beta Actin antibody[GT5512] (GTX629630) dilution: 1:20000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629630 IHC-P Image

beta Actin antibody [GT5512] detects beta Actin protein at cell membrane and cytoplasm in rat adrenal gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
beta Actin antibody [GT5512] (GTX629630) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg mouse brain lysate/extract
D. 30 μg PC-12 whole cell lysate/extract
E. 30 μg rat brain lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 10 μg yeast lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629630 WB Image

The data was published in the journal Sci Rep in 2019.PMID: 30816208

GTX629630 WB Image

The data was published in the journal Front Immunol in 2017.PMID: 29021793

GTX629630 WB Image

The data was published in the journal Oncotarget in 2017.PMID: 28537905

GTX629630 WB Image

The data was published in the journal Sci Rep in 2019.PMID: 30816208

GTX629630 WB Image

The data was published in the journal Genes (Basel) in 2020.PMID: 32085655

GTX629630 WB Image

The data was published in the journal Neuron in 2018.PMID: 29861284

GTX629630 WB Image

The data was published in the journal Sci Rep in 2019.PMID: 30816208

GTX629630 WB Image

The data was published in the journal Front Cell Neurosci in 2018. PMID: 30337858

GTX629630 WB Image

The data was published in the 2021 in Transl Neurosci. PMID: 33708438

GTX629630 WB Image

The data was published in the 2021 in Transl Neurosci. PMID: 33708438

GTX629630 WB Image

The data was published in the journal Cell Death Dis in 2013. PMID: 24008737

GTX629630 WB Image

The data was published in the journal PLoS One in 2014. PMID: 25141004

GTX629630 WB Image

The data was published in the journal PLoS One in 2017. PMID: 28957379

GTX629630 WB Image

The data was published in the journal Toxins (Basel) in 2016. PMID: 27827950

GTX629630 WB Image

The data was published in the journal Toxins (Basel) in 2016. PMID: 27827950

GTX629630 WB Image

The data was published in the journal Front Immunol in 2019. PMID: 31787977

GTX629630 WB Image

The data was published in the journal Cell Discov in 2018. PMID: 30455981

GTX629630 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28169314

REFERENCE

Application Reference

Im JH et al. Int J Mol Sci 2024; 25 (6) Extracellular Vesicles from Cerebrospinal Fluid of Leptomeningeal Metastasis Patients Deliver MiR-21 and Induce Methotrexate Resistance in Lung Cancer Cells.

Lee KT et al. Elife 2024; 12 Comparative interactome analysis of α-arrestin families in human and Drosophila.

Ma L et al. Cell Death Dis 2024; 15 (3) : 229 METTL3-dependent m(6)A modification of PSEN1 mRNA regulates craniofacial development through the Wnt/β-catenin signaling pathway.

YY Chiou et al. J Biol Chem 2024; : 105637 Circadian modulation of glucose utilization via CRY1-mediated repression of Pdk1 expression

Application : WB

Huang-Hobbs E et al. Nature 2023; 619 (7971) : 844-850 Remote neuronal activity drives glioma progression through SEMA4F.

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REVIEW

beta Actin antibody [GT5512] Cat. No. GTX629630

Rating

( Average 4.6 based on 8 users reviews)

Application

Western Blot(WB)		( Average 4.7 based on 7 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)		( Average 4 based on 1 users reviews)

Date : Anonymous submitted on 14-Feb-2023

Score :

Application Tested : WB

Sample Species : Hu

Sample : A549 cell lysates

Amount used : 30μg

Blocking : Blocking solution, 4°C, 10Hr

Primary Antibody : 1:3000, 20°C, 4Hr

Date : Anonymous submitted on 15-Dec-2019

Score :

Application Tested : WB

Sample Species : Ms

Sample : Hepa 1-6 cells

Amount used : 10 μg

Blocking : 5% skim milk in TBST 0.1% Tween-20, 25°C, 1Hr

Primary Antibody : 1:1000, 4°C, 18Hr

Date : Anonymous submitted on 31-May-2018

Score :

Application Tested : WB

Sample Species : Ms

Sample : Brown fat

Primary Antibody : 1:5000, 4°C, 16Hr

Date : Anonymous submitted on 24-Jun-2015

Score :

Application Tested : ICC/IF

Sample Species : Hu

Sample : U87-MG cells

Blocking : 5% BSA in PBST, 25°C, 2Hr

Fixation : 4% PFA

Primary Antibody : 1:200, 4°C, 16Hr

Date : Anonymous submitted on 24-Jun-2015

Score :

Application Tested : WB

Sample Species : Hu

Sample : U87-MG cells

Amount used : 150μg

Blocking : 5% skim milk in PBST, 25°C, 1Hr

Primary Antibody : 1:1000, 4°C, 16Hr

Date : Anonymous submitted on 13-May-2015

Score :

Application Tested : WB

Sample Species : Hu

Sample : HeLa cells

Amount used : 10^4 cells

Blocking : skim milk, 20°C, 0.5Hr

Primary Antibody : 1:10000, 20°C, 16Hr

Date : Anonymous submitted on 13-Jun-2014

Score :

Application Tested : WB

Sample Species : Hu

Sample : HEK293 cells, HeLa cells, MCF-7 cells

Amount used : 5μg

Blocking : 3% skim milk in TBS+0.3% tween20, 25°C, 16Hr

Primary Antibody : 1:10000, 25°C, 1Hr

Lane Description : Lane A:HeLa cells, Lane B:MCF-7 cells, Lane C:HEK293 cells

Date : Anonymous submitted on 07-Apr-2014

Score :

Application Tested : WB

Sample : human Ishikawa cells, rat L6 cells

Amount used : 55μg

Primary Antibody : 1:2000, 4°C, 16Hr

Lane Description : Lane1:human Ishikawa cells, Lane2:rat L6 cells

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Application Reference

Im JH et al. Int J Mol Sci 2024; 25 (6) Extracellular Vesicles from Cerebrospinal Fluid of Leptomeningeal Metastasis Patients Deliver MiR-21 and Induce Methotrexate Resistance in Lung Cancer Cells.

Lee KT et al. Elife 2024; 12 Comparative interactome analysis of α-arrestin families in human and Drosophila.

Ma L et al. Cell Death Dis 2024; 15 (3):229 METTL3-dependent m(6)A modification of PSEN1 mRNA regulates craniofacial development through the Wnt/β-catenin signaling pathway.

YY Chiou et al. J Biol Chem 2024;:105637 Circadian modulation of glucose utilization via CRY1-mediated repression of Pdk1 expression

Application : WB

Huang-Hobbs E et al. Nature 2023; 619 (7971):844-850 Remote neuronal activity drives glioma progression through SEMA4F.

Shen Y et al. Molecules 2023; 29 (1) Transformation of Ginsenosides by Lactiplantibacillus plantarum MB11 Fermentation: Minor Ginsenosides Conversion and Enhancement of Anti-Colorectal Cancer Activity.

K Cermakova et al. Nucleic Acids Res 2023;:gkad1081 Reactivation of the G1 enhancer landscape underlies core circuitry addiction to SWI/SNF

Application : WB

Kadirvelu J et al. bioRxiv 2023; The E3 ubiquitin ligase RNF216 contains a linear ubiquitin chain-determining-like domain that functions to regulate dendritic arborization and dendritic spine type in hippocampal neurons.

Mercado-G?mez OF et al. Brain Sci 2023; 13 (10) Daytime-Restricted Feeding Ameliorates Oxidative Stress by Increasing NRF2 Transcriptional Factor in the Rat Hippocampus in the Pilocarpine-Induced Acute Seizure Model.

Sulistyowati E et al. Int J Mol Sci 2023; 24 (21) Vasculoprotective Potential of Baicalein in Angiotensin II-Infused Abdominal Aortic Aneurysms through Inhibiting Inflammation and Oxidative Stress.

Ballon Romero SS et al. Front Immunol 2023; 14:1213710 Electroacupuncture exerts prolonged analgesic and neuroprotective effects in a persistent dental pain model induced by multiple dental pulp injuries: GABAergic interneurons-astrocytes interaction.

Chiu YS et al. PLoS One 2023; 18 (9):e0291927 Peptide immunization against the C-terminal of alpha-synuclein reduces locomotor activity in mice overexpressing alpha-synuclein.

Jang HJ et al. Exp Mol Med 2023; 55 (8):1720-1733 Thrap3 promotes nonalcoholic fatty liver disease by suppressing AMPK-mediated autophagy.

OF Mercado-Gomez et al. Curr Neurol Neurosci Rep 2023;

Application : WB

Martin-Lopez E et al. J Neurosci 2023; 43 (6):1051-1071 α-Synuclein Pathology and Reduced Neurogenesis in the Olfactory System Affect Olfaction in a Mouse Model of Parkinson's Disease.

JW Chang et al. Biochem Pharmacol 2023; 215:115687 Nesfatin-1 stimulates BMP5 expression and osteoclastogenesis in rheumatoid arthritis

A Guichard et al. Cell Rep 2023; 42 (8):112842 A comprehensive Drosophila resource to identify key functional interactions between SARS-CoV-2 factors and host proteins

Application : WB

Yunjiao Shen et al. Authorea. 2023; Transformation of ginsenosides by Lactiplantibacillus plantarum MB11 fermentation: minor ginsenosides conversion and enhancement of anti-colorectal cancer activity

Application : WB

P Basavaraj et al. Biomed Pharmacother 2023; Elucidation of scandenolone as anti-cancer activity through impairment of the metabolic and signaling vulnerabilities in prostate cancer

Vidyadhara DJ et al. Cell Rep 2023; 42 (3):112231 Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.

E.J. Rodr??guez-Palma et al. PAIN 2023; (164):948-966 Activation of a6-containing GABAA receptors induces antinociception under physiological and pathological conditions

Application : WB

Yeom M et al. Int J Mol Sci 2023; 24 (6)

Application : WB

Reactivity : Human

Didara Z et al. Mol Ther Nucleic Acids 2023; 32:48-60 Inhibition of adenovirus replication by CRISPR-Cas9-mediated targeting of the viral E1A gene.

Application : WB

Reactivity : Human

Kao YT et al. Mol Ther Oncolytics 2023; 28:104-117 Zika virus cleaves GSDMD to disseminate prognosticable and controllable oncolysis in a human glioblastoma cell model.

Ha CM et al. J Am Heart Assoc 2023; 12 (19):e029898 Sustained Increases in Cardiomyocyte Protein O-Linked β-N-Acetylglucosamine Levels Lead to Cardiac Hypertrophy and Reduced Mitochondrial Function Without Systolic Contractile Impairment.

Kim HY et al. Exp Mol Med 2023; 55 (11):2357-2375 PLCγ1 in dopamine neurons critically regulates striatal dopamine release via VMAT2 and synapsin III.

S?nchez-Huerta K et al. Int J Mol Sci 2022; 23 (22) Sucrose Consumption during Late Adolescence Impairs Adult Neurogenesis of the Ventral Dentate Gyrus without Inducing an Anxiety-like Behavior.

Application : WB

Reactivity : Rat

Biswas S et al. Heliyon 2022; 8 (11):e11656 PEITC: A resounding molecule averts metastasis in breast cancer cells in vitro by regulating PKCδ/Aurora A interplay.

Sun L et al. Mar Drugs 2022; 20 (11) Astaxanthin Exerts Immunomodulatory Effect by Regulating SDH-HIF-1α Axis and Reprogramming Mitochondrial Metabolism in LPS-Stimulated RAW264.7 Cells.

Application : WB

Reactivity : Mouse

Engel AL et al. Exp Eye Res 2022; 215:108899 Extracellular matrix dysfunction in Sorsby patient-derived retinal pigment epithelium.

Jia S et al. J Neurophysiol 2022; 128 (6):1383-1394 Chronic intermittent hypoxia enhances glycinergic inhibition in nucleus tractus solitarius.

Hung SY et al. Front Cell Neurosci 2022; 16:880267 Electroacupuncture improves TBI dysfunction by targeting HDAC overexpression and BDNF-associated Akt/GSK-3β signaling.

Huang HC et al. Int J Mol Sci 2022; 23 (13) Effects of PCSK-9 Inhibition by Alirocumab Treatments on Biliary Cirrhotic Rats.

Arlene J George et al. iScience 2022; 25 (6):104386 The E3 ubiquitin ligase RNF216/TRIAD3 is a key coordinator of the hypothalamic-pituitary-gonadal axis.

Han-Hsiang Chen et al. J Biol Chem 2022; 298 (6):101957 Development of antiviral carbon quantum dots that target the Japanese encephalitis virus envelope protein.

Shu-Mei Chen et al. Int J Mol Sci 2022; 23 (12) Microtube Array Membrane Encapsulated Cell Therapy: A Novel Platform Technology Solution for Treatment of Alzheimer's Disease.

Application : WB

Reactivity : Mouse

Shaozhen Feng et al. J Biol Chem 2022; 298 (5):101816 Downregulation of the endothelial histone demethylase JMJD3 is associated with neointimal hyperplasia of arteriovenous fistulas in kidney failure.

Application : WB

Reactivity : Mouse

Erica L Gorenberg et al. PLoS Biol 2022; 20 (3):e3001590 Identification of substrates of palmitoyl protein thioesterase 1?highlights roles of depalmitoylation in disulfide bond formation and synaptic function.

Oluwadarasimi Fadeyibi et al. J Endocr Soc 2022; 6 (5):bvac030 The Role of Lipid Rafts and Membrane Androgen Receptors in Androgen's Neurotoxic Effects.

Application : WB

Reactivity : Rat

Hage A et al. Cell Rep 2022; 38 (10):110434 The RNA helicase DHX16 recognizes specific viral RNA to trigger RIG-I-dependent innate antiviral immunity.

Application : WB

Reactivity : Human

Huang SH et al. Int J Mol Sci 2022; 23 (3) Alpha-Lipoic Acid Inhibits Spontaneous Diabetes and Autoimmune Recurrence in Non-Obese Diabetic Mice by Enhancing Differentiation of Regulatory T Cells and Showed Potential for Use in Cell Therapies for the Treatment of Type 1 Diabetes.

Application : WB

Chen PM et al. Int J Mol Sci 2022; 23 (1) Downregulation of Methionine Cycle Genes MAT1A and GNMT Enriches Protein-Associated Translation Process and Worsens Hepatocellular Carcinoma Prognosis.

Application : WB

Reactivity : Human

Z??iga-Romero A et al. Pharmaceuticals 2022; 15 (1):67 GPR55 and GPR119 Receptors Contribute to the Processing of Neuropathic Pain in Rats.

DeJong CS et al. Development 2021; 148 (15) The atypical RNA-binding protein Taf15 regulates dorsoanterior neural development through diverse mechanisms in Xenopus tropicalis.

Yu-Jen Chiu et al. In Vivo 2021; 35 (6):3181-3191 Curcumin Derivative MTH-3 Regulates Palmitate-induced Insulin Resistance in Mouse Myoblast C₂C₁₂ Cells.

Application : WB

Reactivity : Mouse

Xin Li et al. Transl Neurosci 2021; 12 (1):103-113 Inhibition of lncRNA H19/miR-370-3p pathway mitigates neuronal apoptosis in an in vitro model of spinal cord injury (SCI).

Application : WB

Reactivity : Rat

Mara A Guzm?n-Ruiz et al. BMC Neurosci 2021; 22 (1):14 Protective effects of intracerebroventricular adiponectin against olfactory impairments in an amyloid β_1-42 rat model.

Application : WB

Reactivity : Mouse

Yen-Yun Wang et al. Oncol Lett 2021; 21 (6):450 Excision repair cross-complementing group 2 upregulation is a potential predictive biomarker for oral squamous cell carcinoma recurrence.

Application : WB, IHC-P

Reactivity : Human

Lin-Sheng Yu et al. Cancers (Basel) 2021; 13 (16) Tissue Architecture Influences the Biological Effectiveness of Boron Neutron Capture Therapy in In Vitro/In Silico Three-Dimensional Self-Assembly Cell Models of Pancreatic Cancers.

Application : WB

Reactivity : Human

Henry B C Taylor et al. Cell Rep 2021; 36 (9):109638 Long-term depression links amyloid-β to the pathological hyperphosphorylation of tau.

Application : WB

Jeon SH et al. Nutrients 2021; 13 (12) Oyster Hydrolysates Attenuate Muscle Atrophy via Regulating Protein Turnover and Mitochondria Biogenesis in C2C12 Cell and Immobilized Mice.

Application : WB

Reactivity : Mouse

Anderson R et al. FASEB J 2021; 35 (11):e21990 eIF2A-knockout mice reveal decreased life span and metabolic syndrome.

Application : WB

Reactivity : Mouse

Chen IC et al. Pharmaceuticals (Basel) 2021; 14 (10) Corylin Ameliorates LPS-Induced Acute Lung Injury via Suppressing the MAPKs and IL-6/STAT3 Signaling Pathways.

Application : WB

Reactivity : Mouse

Lee SH et al. PLoS One 2021; 16 (8):e0254587 Three month inhalation exposure to low-level PM2.5 induced brain toxicity in an Alzheimer's disease mouse model.

Application : WB

Reactivity : Mouse

Chang YC et al. Nat Commun 2021; 12 (1):4258 VCP maintains nuclear size by regulating the DNA damage-associated MDC1-p53-autophagy axis in Drosophila.

Application : WB

Reactivity : Drosophila

Garc?a G et al. Behav Brain Res 2021; 413:113446 TREK-1 potassium channels participate in acute and long-lasting nociceptive hypersensitivity induced by formalin in rats.

Nguyen ATM et al. Sci Rep 2021; 11 (1):13694 Electroacupuncture prevents cocaine-induced conditioned place preference reinstatement and attenuates ΔFosB and GluR2 expression.

Application : WB

Reactivity : Mouse

Hsieh YC et al. Cell Mol Gastroenterol Hepatol 2021; 12 (3):813-838 (Pro)renin Receptor Knockdown Attenuates Liver Fibrosis Through Inactivation of ERK/TGF-β1/SMAD3 Pathway.

Application : WB

Reactivity : Mouse

Hou Y et al. Biochem Biophys Res Commun 2021; 550:22-29 LF-rTMS ameliorates social dysfunction of FMR1^-/- mice via modulating Akt/GSK-3β signaling.

Application : WB

Reactivity : Mouse

Zhao J et al. Transl Res 2021; Mitochondria transplantation protects traumatic brain injury via promoting neuronal survival and astrocytic BDNF.

Mara A. Guzm?n-Ruiz et al. BMC Neuroscience 2021; 22:14 Protective effects of intracerebroventricular adiponectin against olfactory impairments in an amyloid β1–42 rat model.

Bl?zquez AB et al. PLoS Negl Trop Dis 2021; 15 (1):e0009072 Relevance of oxidative stress in inhibition of eIF2 alpha phosphorylation and stress granules formation during Usutu virus infection.

Application : WB

Mondal UK et al. Int J Mol Sci 2021; 22 (3) PEG Linker Length Strongly Affects Tumor Cell Killing by PEGylated Carbonic Anhydrase Inhibitors in Hypoxic Carcinomas Expressing Carbonic Anhydrase IX.

Application : WB

Reactivity : Human

Ma T et al. Life Sci 2021; 274:119291 Imperatorin alleviated endometriosis by inhibiting the activation of PI3K/Akt/NF-κB pathway in rats.

Application : WB

Chang Y et al. J Med Food 2021; 24 (3):209-217 Albanin A, Derived from the Root Bark of Morus alba L., Depresses Glutamate Release in the Rat Cerebrocortical Nerve Terminals via Ca²⁺/Calmodulin/Adenylate Cyclase 1 Suppression.

Application : WB

Lee PC et al. Mol Nutr Food Res 2021; 65 (3):e2000937 Active Vitamin D₃ Treatment Attenuated Bacterial Translocation via Improving Intestinal Barriers in Cirrhotic Rats.

Application : WB

Reactivity : Rat

Kim SH et al. Proc Natl Acad Sci U S A 2021; 118 (3) Prediction of Alzheimer's disease-specific phospholipase c gamma-1 SNV by deep learning-based approach for high-throughput screening.

Application : WB

Reactivity : Mouse

HJ Jang et al. FASEB J 2020; 34 (1):1270-1287 Glucosylceramide synthase regulates adipo-osteogenic differentiation through synergistic activation of PPARγ with GlcCer

Application : WB

Lamothe SM et al. Elife 2020; 9 Control of Slc7a5 sensitivity by the voltage-sensing domain of Kv1 channels.

Brahma MK et al. J Am Heart Assoc 2020; 9 (15):e013039 Increased Glucose Availability Attenuates Myocardial Ketone Body Utilization.

Application : WB

Reactivity : Mouse

German? A et al. Int J Mol Sci 2020; 21 (16) Expression and Localization of BDNF/TrkB System in the Zebrafish Inner Ear.

Valvezan AJ et al. JCI Insight 2020; 5 (7) IMPDH inhibitors for antitumor therapy in tuberous sclerosis complex.

Ballon Romero SS et al. Int J Mol Sci 2020; 21 (7) Analgesic and Neuroprotective Effects of Electroacupuncture in a Dental Pulp Injury Model-A Basic Research.

Ma L et al. J Cell Mol Med 2020; (Epub) Identification of novel susceptibility loci for non-syndromic cleft lip with or without cleft palate.

Application : WB

Reactivity : Zebrafish

Shin JE et al. Nutrients 2020; 12 (11) Soluble Whey Protein Hydrolysate Ameliorates Muscle Atrophy Induced by Immobilization via Regulating the PI3K/Akt Pathway in C57BL/6 Mice.

Application : WB

Reactivity : Mouse

Wu PS et al. Biomedicines 2020; 8 (11) 8-Hydroxydaidzein, an Isoflavone from Fermented Soybean, Induces Autophagy, Apoptosis, Differentiation, and Degradation of Oncoprotein BCR-ABL in K562 Cells.

Application : WB

Reactivity : Human

Zou W et al. Environ Sci Technol 2020; 54 (21):13888-13898 Photo-Oxidative Degradation Mitigated the Developmental Toxicity of Polyamide Microplastics to Zebrafish Larvae by Modulating Macrophage-Triggered Proinflammatory Responses and Apoptosis.

Application : WB

Reactivity : Zebrafish

Chen PY et al. Biochem Pharmacol 2020; 182:114294 Increased leptin-b expression and metalloprotease expression contributed to the pyridoxine-associated toxicity in zebrafish larvae displaying seizure-like behavior.

Application : WB

Reactivity : Zebrafish

Homma T et al. Sci Rep 2020; 10 (1):17934 Testis-specific peroxiredoxin 4 variant is not absolutely required for spermatogenesis and fertility in mice.

Application : WB

Reactivity : Mouse

Homma T et al. Free Radic Biol Med 2020; (Epub) Defective biosynthesis of ascorbic acid in Sod1-deficient mice results in lethal damage to lung tissue.

Application : WB

Reactivity : Mouse

Geng X et al. Biochem Pharmacol 2020; 182:114226 Cardiac glycosides inhibit cancer through Na/K-ATPase-dependent cell death induction.

Application : WB

Reactivity : Human

Franco-Enz?stiga ? et al. J Neurochem 2020; (Epub) Sex-dependent pronociceptive role of spinal α₅ -GABA_A receptor and its epigenetic regulation in neuropathic rodents.

Application : WB

Reactivity : Rat

Yeom M et al. Chem Res Toxicol 2020; 33 (8):2120-2129 Three Human Pol ι Variants with Impaired Polymerase Activity Fail to Rescue H₂O₂ Sensitivity in POLI-Deficient Cells.

Application : WB

Reactivity : Human

Jim?nez A et al. Neurochem Res 2020; 45 (8):1781-1790 Olfactory Dysfunction in Diabetic Rats is Associated with miR-146a Overexpression and Inflammation.

Application : WB

Reactivity : Rat

Han Z et al. J Biol Chem 2020; 295 (25):8596-8601 Angiomotin regulates budding and spread of Ebola virus.

Application : WB

Reactivity : Human

Jo CS et al. Theranostics 2020; 10 (9):3880-3891 A novel function of Prohibitin on melanosome transport in melanocytes.

Application : WB

Reactivity : Mouse

Hwang B et al. Genes Cells 2020; 25 (6):413-426 Multimeric conformation of type III intermediate filaments but not the filamentous conformation exhibits high affinity to lipid bilayers.

Application : WB

Reactivity : Human

Hao Z et al. Mol Cell 2020; 78 (3):382-395.e8 N 6-Deoxyadenosine Methylation in Mammalian Mitochondrial DNA.

Application : WB

Reactivity : Human

Wang C et al. Cell Rep 2020; 30 (6):1780-1797.e6 Secreted Pyruvate Kinase M2 Promotes Lung Cancer Metastasis through Activating the Integrin Beta1/FAK Signaling Pathway.

Application : WB

Reactivity : Human

Chemmarappally JM et al. Sci Rep 2020; 10 (1):2767 A co-culture nanofibre scaffold model of neural cell degeneration in relevance to Parkinson's disease.

Application : WB

Reactivity : Human

Srivastava SP et al. Genes (Basel) 2020; 11 (2) Inhibition of Angiotensin-Converting Enzyme Ameliorates Renal Fibrosis by Mitigating DPP-4 Level and Restoring Antifibrotic MicroRNAs.

Application : WB

Reactivity : Human

Lu SY et al. Hum Gene Ther 2020; (Epub) Lentivirus-mediated hFGF21 Stable Expression in Liver of Diabetic Rats Model and It's Anti-diabetic Effect Observation.

Application : WB

Reactivity : Human

Tahara T et al. Bioorg Med Chem 2020; 28 (6):115347 STAT3 inhibitory activity of naphthoquinones isolated from Tabebuia avellanedae.

Application : WB

Reactivity : Human

Bravo-Hernandez M et al. Nat Med 2020; 26 (1):118-130 Spinal subpial delivery of AAV9 enables widespread gene silencing and blocks motoneuron degeneration in ALS.

Application : WB

Reactivity : Mouse

Han Z et al. PLoS Pathog 2020; 16 (1):e1008231 Modular mimicry and engagement of the Hippo pathway by Marburg virus VP40: Implications for filovirus biology and budding.

Application : WB

Reactivity : Human

Hsu HP et al. J Cancer 2020; 11 (1):213-228 Knockdown of serine/threonine-protein kinase 24 promotes tumorigenesis and myeloid-derived suppressor cell expansion in an orthotopic immunocompetent gastric cancer animal model.

Application : WB

El-Daher MT et al. Front Immunol 2019; 10:2592 Chronic Intestinal Pseudo-Obstruction and Lymphoproliferative Syndrome as a Novel Phenotype Associated With Tetratricopeptide Repeat Domain 7A Deficiency.

Hung JJ et al. Sci Rep 2019; 9 (1):2991 Overexpression of Aiolos promotes epithelial-mesenchymal transition and cancer stem cell-like properties in lung cancer cells.

Yao F et al. Oncol Lett 2019; 18 (5):4825-4833 Primary impact of Gli1 on radioresistance in esophageal cancer.

Application : WB

Reactivity : Human

Zuo X et al. J Biol Chem 2019; (Epub) Primary cilia and the exocyst are linked to urinary extracellular vesicle production and content.

Application : WB

Reactivity : Dog

YingGuo et al. CJD 2019; (Epub) MicroRNA-15a inhibits GLUT4 translocation and impairs glucose metabolism in L6 skeletal muscle via targeting of VAP-A.

Application : WB

Liu WL et al. Molecules 2019; 22 (6):pii: E929 Curcumin Inhibits LIN-28A through the Activation of miRNA-98 in the Lung Cancer Cell Line A549.

Application : WB

Reactivity : Human

Chang W et al. J Agric Food Chem 2019; 67 (27):7684-7693 Protective Effect of Caffeic Acid against Alzheimer's Disease Pathogenesis via Modulating Cerebral Insulin Signaling, β-Amyloid Accumulation, and Synaptic Plasticity in Hyperinsulinemic Rats.

Application : WB

Reactivity : Rat

Kjolby RAS et al. Development 2019; (Epub) Integration of Wnt and FGF signaling in the Xenopus gastrula at TCF and Ets binding sites shows the importance of short range repression in patterning the marginal zone.

Sonia L. Evagelou et al. bioRxiv 2019; (Epub) DEAD-box protein family member DDX28 is a negative regulator of HIF-2α and eIF4E2-directed hypoxic translation.

Application : WB

Reactivity : Human

Dyikanov DT et al. Tissue Eng Part C Methods 2019; 25 (3):168-175 Optimization of CRISPR/Cas9 Technology to Knock Out Genes of Interest in Aneuploid Cell Lines.

Application : WB

Reactivity : Human

Shih JC et al. FASEB J 2019;:fj201801898RR Unveiling the role of microRNA-7 in linking TGF-β-Smad-mediated epithelial-mesenchymal transition with negative regulation of trophoblast invasion.

Application : WB

Reactivity : Human

Hu CM et al. Cell Metab 2019; (Epub) High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells.

Application : WB

Reactivity : Human

Timpano S et al. FASEB J 2019;:fj201802279R Physioxic human cell culture improves viability, metabolism, and mitochondrial morphology while reducing DNA damage.

Application : WB

Reactivity : Human

Lee YC et al. Int J Med Sci 2018; 15 (9):953-960 Manual Acupuncture Relieves Bile Acid-Induced Itch in Mice: The Role of Microglia and TNF-α.

Qi J et al. Front Mol Neurosci 2018; 11:281 Sulbactam Protects Hippocampal Neurons Against Oxygen-Glucose Deprivation by Up-Regulating Astrocytic GLT-1 via p38 MAPK Signal Pathway.

Yang LQ et al. Front Cell Neurosci 2018; 12:348 Hypoxia Delays Oligodendrocyte Progenitor Cell Migration and Myelin Formation by Suppressing Bmp2b Signaling in Larval Zebrafish.

Pang YL et al. Animal Model Exp Med 2018; 1 (3):203-211 Comparative medical characteristics of ZDF-T2DM rats during the course of development to late stage disease.

Zhao RQ et al. Front Pharmacol 2018; 9:1545 Y₆, an Epigallocatechin Gallate Derivative, Reverses ABCG2-Mediated * Resistance.

Application : WB

Reactivity : Human

El-Daher MT et al. Cell Discov 2018; 4:61 Tetratricopeptide repeat domain 7A is a nuclear factor that modulates transcription and chromatin structure.

Application : WB

Reactivity : Human

Carla Garza-Lombo et al. Neurochemistry International 2018; (Epub) Systemic L-buthionine-S-R-sulfoximine administration modulates glutathione homeostasis via NGF/TrkA and mTOR signaling in the cerebellum.

Application : WB

Reactivity : Mouse

Yun?Li Pang et al. Animal Models and Experimental Medicine 2018; 1 (3):203-211 Comparative medical characteristics of ZDF-T2DM rats during the course of development to late stage disease.

Application : WB

Reactivity : Rat

Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.

Application : IP

Reactivity : Human

Surrey V et al. Neuroscience 2018; 386:24-40 Impaired Local Translation of β-actin mRNA in Ighmbp2-Deficient Motoneurons: Implications for Spinal Muscular Atrophy with respiratory Distress (SMARD1).

Lee Y.C. et al. Int J Med Sci 2018; 15 (9):953-960 Manual acupuncture relieves bile acid-induced itch in mice: the role of microglia and TNF-α.

Application : WB

Reactivity : Mouse

Gao J et al. Cancer Biomark 2018; (Epub) Targetedregulationof STAT3 by miR-29a in mediating * resistance of nasopharyngeal carcinoma cell line CNE-1.

Reactivity : Human

Wall MJ et al. Neuron 2018; (Epub) The Temporal Dynamics of Arc Expression Regulate Cognitive Flexibility.

Application : WB

Reactivity : Mouse

Ahmed M. Shabana et al. ACS Appl. Mater. Interfaces 2018; (Epub) ph-sensitive multiligand gold nanoplatform targeting carbonic anhydrase ix enhances the delivery of * to hypoxic tumor spheroids and overcomes the hypoxia-induced chemoresistance.

Application : WB

Reactivity : Human

Singh AK et al. Mol Metab 2018; (Epub) Brown adipose tissue derived ANGPTL4 controls glucose and lipid metabolism and regulates thermogenesis.

Application : WB

Reactivity : Mouse

Hong S et al. Hum Mol Genet 2018; (Epub) Loss-of-function mutations in FGF8 can be independent risk factors for holoprosencephaly.

Application : WB

Reactivity : Human

Wang DW et al. Eur. Rev. Med. Pharmacol. Sci. 2018; 22:1241-1248 MiR-16 inhibits pituitary adenoma cell proliferation via the suppression of ERK/MAPK signal pathway.

Application : WB

Reactivity : Human

Tamarand Lee Darling et al. Front Immunol 2017; 8:1197 Intracellular Crosslinking of Filoviral Nucleoproteins with Xintrabodies Restricts Viral Packaging.

Application : WB

Reactivity : Monkey

Chuang TY et al. Oncotarget 2017; 8 (30):49735-49748 Suppression of LPS-induced inflammatory responses by the hydroxyl groups of dexamethasone.

Application : WB

Reactivity : Mouse

Roy D et al. Sci Rep 2017; 7:41977 Loss of HSulf-1: The Missing Link between Autophagy and Lipid Droplets in Ovarian Cancer.

Hurwitz SN et al. J Virol 2017; (Epub) Tetraspanin CD63 bridges autophagic and endosomal processes to regulate exosomal secretion and intracellular signaling of Epstein-Barr virus LMP1.

Application : WB

Reactivity : Human

Grimaldo L et al. PLoS One 2017; 12 (9):e0185289 Involvement of Parkin in the ubiquitin proteasome system-mediated degradation of N-type voltage-gated Ca2+ channels.

Application : WB

Reactivity : Human

Fang F et al. Sci Rep 2017; 7 (1):3868 Synuclein impairs trafficking and signaling of BDNF in a mouse model of Parkinson's disease.

Application : WB

Reactivity : Mouse

Coble JL et al. Hum Mol Genet 2017; (Epub) Identification of a rare LAMB4 variant associated with familial diverticulitis through exome sequencing.

Application : WB

Reactivity : Human

Salinas-Abarca AB et al. Eur J Pharmacol 2017; 797:83-93 Formalin injection produces long-lasting hypersensitivity with characteristics of neuropathic pain.

Application : WB

Reactivity : Rat

Shahrour MA et al. Neurogenetics 2016; 17 (4):227-232 PARP10 deficiency manifests by infantile neurodegeneration and DNA repair defect.

Application : WB

Reactivity : Human

Hsieh JT et al. Urology 2016; 91:242.e1-9 Epigallocatechin Gallate Attenuates Partial Bladder Outlet Obstruction-induced Bladder Injury via Suppression of Endoplasmic Reticulum Stress-related Apoptosis-In Vivo Study.

Application : WB

Reactivity : Rat

Chen CY et al. Toxins (Basel) 2016; 8 (11) Tenuifolide B from Cinnamomum tenuifolium Stem Selectively Inhibits Proliferation of Oral Cancer Cells via Apoptosis, ROS Generation, Mitochondrial Depolarization, and DNA Damage.

Application : WB

Reactivity : Human

Kotoh J et al. J Vet Med Sci 2016; Plekhs1 and Prdx3 are candidate genes responsible for mild hyperglycemia associated with obesity in a new animal model of F344-fa-nidd6 rat.

Application : WB

Reactivity : Rat

Ohta S et al. J Proteome Res 2016; 15 (9):3331-41 Identification of Mitosis-Specific Phosphorylation in Mitotic Chromosome-Associated Proteins.

Wu YY et al. Am J Cancer Res 2016; 6 (6):1253-70 CASZ1 is a novel promoter of metastasis in ovarian cancer.

Application : WB

Reactivity : Human

Kjolby RA et al. Dev Biol 2016; (Epub) Genome-wide identification of Wnt/β-catenin transcriptional targets during Xenopus gastrulation.

Application : WB

Reactivity : Xenopus

Choe KN et al. EMBO Rep 2016; (Epub) HUWE1 interacts with PCNA to alleviate replication stress.

Application : WB

Reactivity : Human

Akocak S et al. J Med Chem 2016; (Epub) PEGylated Bis-Sulfonamide Carbonic Anhydrase Inhibitors Can Efficiently Control the Growth of Several Carbonic Anhydrase IX-Expressing Carcinomas.

Application : WB

Reactivity : Human

Alshogran OY et al. Drug Metab Dispos 2015; 43 (1):100-6 Effect of experimental kidney disease on the functional expression of hepatic reductases.

Application : WB

Reactivity : Rat

Zhong X et al. Endocrinology 2015; 156 (9):3157-68 The MicroRNAs in the Pathogenesis of Metabolic Memory.

Application : WB

Reactivity : Human

Chang WC et al. Food Res Int 2015; (Epub) caffeic acid improves memory impairment and brain glucose metabolism via ameliorating cerebral * and leptin signaling pathways in high-fat diet-induced hyper*emic rats.

Application : WB

Reactivity : Rat

Hou HH et al. Respir Res 2014; 15:106 PlGF mediates neutrophil elastase-induced airway epithelial cell apoptosis and emphysema.

Application : WB

Reactivity : Human

Noomhorm N et al. J Pharmacol Sci 2014; 125 (4):375-85 in vitro and in vivo effects of xanthorrhizol on human breast cancer mcf-7 cells treated with *.

Application : WB

Reactivity : Human

Huang SY et al. PLoS One 2014; 9 (8):e104414 A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema.

Application : WB

Reactivity : Zebrafish

Alberio T et al. Biochim Biophys Acta 2014; 1842 (9):1816-1822 Altered * homeostasis differentially affects mitochondrial voltage-dependent anion channels turnover.

Application : WB

Reactivity : Human

Hou HH et al. Cell Death Dis 2013; 4:e793 Elastase induced lung epithelial cell apoptosis and emphysema through placenta growth factor.

Application : WB

Reactivity : Mouse

SDS
PBS.pdf

Package	List Price ($)
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25 μl	$ 169
Qty Custom Conjugations

beta Actin antibody [GT5512]

Cat. No. GTX629630

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beta Actin antibody [GT5512] Cat. No. GTX629630

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