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p27 Kip1 antibody

KO/KD Orthogonal IP/MS
Anti-p27 Kip1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100446
Anti-p27 Kip1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100446
Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446
Anti-p27 Kip1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100446
Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446
Anti-p27 Kip1 antibody used in Immunoprecipitation (IP). GTX100446
Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446
Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446
Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446
Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446
Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446

Cat. No. GTX100446

Reference 28
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all p27 Kip1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Product Note

IP/MS validation was supported by references (PMID:30377371)

Predict Reactivity

Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949954

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase inhibitor 1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p27 Kip1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100446

GTX100446 IHC-P Image

Immunohistochemical staining of human ulcerative colitis tissue using anti-p27 Kip1 antibody (GTX100446).

Anti-p27 Kip1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100446

GTX100446 ICC/IF Image

p27 Kip1 antibody detects p27 Kip1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p27 Kip1 protein stained by p27 Kip1 antibody (GTX100446) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446

GTX100446 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (GTX100446) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-p27 Kip1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100446

GTX100446 IHC-Fr Image

p27 Kip1 antibody detects p27 Kip1 protein at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: p27 Kip1 protein stained by p27 Kip1 antibody (GTX100446) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446

GTX100446 WB Image

KO/KD

Wild-type (WT) and p27 Kip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (GTX100446) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-p27 Kip1 antibody used in Immunoprecipitation (IP). GTX100446

GTX100446 IP Image

Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using 5 μg of p27 Kip1 antibody (GTX100446).
Western blot analysis was performed using p27 Kip1 antibody (GTX100446).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446

GTX100446 WB Image

p27 Kip1 antibody detects p27 Kip1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (GTX100446) diluted at 1:1000.

Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446

GTX100446 WB Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2018. PMID: 29522490

Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446

GTX100446 WB Image

Orthogonal

The data was published in the journal EMBO J in 2018. PMID: 29084722

Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446

GTX100446 WB Image

Orthogonal

The data was published in the journal Cell Death Dis in 2014.PMID: 25429617

Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446

GTX100446 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28134285

REFERENCE

Application Reference

Liao YC et al. Mol Ther Nucleic Acids 2023; 33 : 351-366 Tumor targeting and therapeutic assessments of RNA nanoparticles carrying α9-nAChR aptamer and anti-miR-21 in triple-negative breast cancers.
Ming-Lin Tsai et al. FEBS Open Bio 2022; 12 (5) : 993-1005 CRISPR-mediated knockout of VEGFR2/KDR inhibits cell growth in a squamous thyroid cancer cell line.
Application : WB
Reactivity : Human
Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Mao BH et al. Part Fibre Toxicol 2022; 19 (1) : 6 Use of an in silico knowledge discovery approach to determine mechanistic studies of silver nanoparticles-induced toxicity from in vitro to in vivo.
Application : WB
Reactivity : Human
Aitken TJ et al. Biol Cell 2021; 113 (12) : 507-521 Decreased proliferation of aged rat beta cells corresponds with enhanced expression of the cell cycle inhibitor p27(KIP1).
Application : WB
Reactivity : Rat
Show More +
Submit a Reference

REVIEW

p27 Kip1 antibody Cat. No. GTX100446

Rating
( Average 3 based on 1 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-p27 Kip1 antibody used in Western Blot (WB). GTX100446
Date : Anonymous submitted on 21-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells,Jurkat cells
Amount used : 50μg
Blocking : 8% BSA in 1X TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Liao YC et al. Mol Ther Nucleic Acids 2023; 33:351-366 Tumor targeting and therapeutic assessments of RNA nanoparticles carrying α9-nAChR aptamer and anti-miR-21 in triple-negative breast cancers.
Ming-Lin Tsai et al. FEBS Open Bio 2022; 12 (5):993-1005 CRISPR-mediated knockout of VEGFR2/KDR inhibits cell growth in a squamous thyroid cancer cell line.
Application : WB
Reactivity : Human
Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Mao BH et al. Part Fibre Toxicol 2022; 19 (1):6 Use of an in silico knowledge discovery approach to determine mechanistic studies of silver nanoparticles-induced toxicity from in vitro to in vivo.
Application : WB
Reactivity : Human
Aitken TJ et al. Biol Cell 2021; 113 (12):507-521 Decreased proliferation of aged rat beta cells corresponds with enhanced expression of the cell cycle inhibitor p27(KIP1).
Application : WB
Reactivity : Rat
Ching-Ling Lin et al. Biomol Ther (Seoul) 2021; 29 (5):551-561 Platelet-Derived Growth Factor Receptor-α Subunit Targeting Suppresses Metastasis in Advanced Thyroid Cancer In Vitro and In Vivo.
Application : WB
Reactivity : Human
Jia-Ming Chang et al. Int J Mol Sci 2021; 22 (18) 9-O-Terpenyl-Substituted Berberrubine Derivatives Suppress Tumor Migration and Increase Anti-Human Non-Small-Cell Lung Cancer Activity.
Application : WB
Reactivity : Human
Chang MC et al. Int Endod J 2021; (Epub) Effect of butyrate, a bacterial by-product, on the viability and ICAM-1 expression/production of human vascular endothelial cells: Role in infectious pulpal/periapical diseases.
Application : WB
Reactivity : Human
Alza L et al. Cancers (Basel) 2020; 12 (5) FAK Inhibition Induces Glioblastoma Cell Senescence-Like State through p62 and p27.
Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.
Application : WB
Reactivity : Human
Yeh HT et al. Eur J Pharmacol 2019; 863:172658 Flavopereirine induces cell cycle arrest and apoptosis via the AKT/p38 MAPK/ERK1/2 signaling pathway in human breast cancer cells.
Application : WB
Reactivity : Human
Wang S et al. Cancer Lett 2019; 465:12-23 MicroRNA-17 acts as a tumor chemosensitizer by targeting JAB1/CSN5 in triple-negative breast cancer.
Application : IHC
Reactivity : Human
Li HY et al. J Agric Food Chem 2019; 67 (7):1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Application : WB
Reactivity : Mouse
Scully KM et al. Cell Mol Gastroenterol Hepatol 2018; 6 (2):181-198 E47 Governs the MYC-CDKN1B/p27 KIP1-RB Network to Growth Arrest PDA Cells Independent of CDKN2A/p16 INK4A and Wild-Type p53.
Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Wu CH et al. Int J Mol Sci 2018; 19 (3) Suppression of Cell Growth, Migration and Drug Resistance by Ethanolic Extract of Antrodia cinnamomea in Human Lung Cancer A549 Cells and C57BL/6J Allograft Tumor Model.
Application : WB
Reactivity : Human
Mlcochova P et al. EMBO J 2017; (Epub) DNA damage induced by topoisomerase inhibitors activates SAMHD1 and blocks HIV-1 infection of macrophages.
Application : WB
Reactivity : Human
Liu YH et al. Sci Rep 2017; 7:41437 Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
Application : WB
Reactivity : Human
Xu YM et al. Oncotarget 2016; 7 (5):6146-58 Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Chen CC et al. PLoS One 2016; 11 (12):e0168092 Viscolin Inhibits In Vitro Smooth Muscle Cell Proliferation and Migration and Neointimal Hyperplasia In Vivo.
Application : WB
Reactivity : Human
Chang MC et al. PLoS One 2016; 11 (11):e0165438 Effect of Butyrate on Collagen Expression, Cell Viability, Cell Cycle Progression and Related Proteins Expression of MG-63 Osteoblastic Cells.
Lu CH et al. Pharmacol Res 2016; 115:288-298 Honokiol, a potential therapeutic agent, induces cell cycle arrest and program cell death in vitro and in vivo in human thyroid cancer cells.
Application : WB
Reactivity : Human
Pan CH et al. Toxicol Appl Pharmacol 2014; K20E, an oxidative-coupling compound of methyl caffeate, exhibits anti-angiogenic activities through down-regulations of VEGF and VEGF receptor-2.
Application : WB
Reactivity : Mouse
Chang HY et al. Cell Death Dis 2014; 5:e1540 Combination therapy targeting ectopic ATP synthase and 26S proteasome induces ER stress in breast cancer cells.
Application : WB
Reactivity : Human
Kao YT et al. KAOHSIUNG J MED SCI 2014; involvement of p38 mitogen-activated protein kinase in acquired *-resistant human urothelial carcinoma sublines
Ohmachi Y et al. J Toxicol Pathol 2013; 26 (4):413-7 Two Cases of Metastatic Parathyroid Carcinoma in Male C3H Mice Following Irradiation.
Application : IHC-P
Reactivity : Mouse
Liu FC et al. Phytomedicine 2013; 20 (10):874-82 Elucidating the inhibitory mechanisms of the ethanolic extract of the fruiting body of the mushroom Antrodia cinnamomea on the proliferation and migration of murine leukemia WEHI-3 cells and their tumorigenicity in a BALB/c allograft tumor model.
Application : WB
Reactivity : Mouse
Zhang YY et al. Mol Endocrinol 2012; 26 (5):798-808 Down-regulation of type I Runx2 mediated by * is required for 3T3-L1 adipogenesis.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169