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53BP1 antibody [HL275]

Recombinant antibody KO/KD Orthogonal
Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479
Anti-53BP1 antibody [HL275] used in IHC (Paraffin sections) (IHC-P). GTX635479
Anti-53BP1 antibody [HL275] used in IHC (Paraffin sections) (IHC-P). GTX635479
Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479
Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479
Anti-53BP1 antibody [HL275] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635479
Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479
Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479
Anti-53BP1 antibody [HL275] used in IHC (Paraffin sections) (IHC-P). GTX635479
Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479
Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

Cat. No. GTX635479

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL275

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all 53BP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

214 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human 53BP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888536

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 binding protein 1 , 53BP1 , TDRD30 , p202 , p53BP1

Cellular Localization

Nucleus

Background

This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

GTX635479 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-53BP1 antibody [HL275] used in IHC (Paraffin sections) (IHC-P). GTX635479

GTX635479 IHC-P Image

53BP1 antibody [HL275] detects 53BP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
53BP1 stained by 53BP1 antibody [HL275] (GTX635479) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-53BP1 antibody [HL275] used in IHC (Paraffin sections) (IHC-P). GTX635479

GTX635479 IHC-P Image

53BP1 antibody [HL275] detects 53BP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
53BP1 stained by 53BP1 antibody [HL275] (GTX635479) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

GTX635479 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

GTX635479 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-53BP1 antibody [HL275] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635479

GTX635479 ICC/IF Image

53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 53BP1 stained by 53BP1 antibody [HL275] (GTX635479) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

GTX635479 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

GTX635479 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-53BP1 antibody [HL275] used in IHC (Paraffin sections) (IHC-P). GTX635479

GTX635479 IHC-P Image

53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
53BP1 stained by 53BP1 antibody [HL275] (GTX635479) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

GTX635479 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

GTX635479 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL275] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Liu G et al. Front Oncol 2021; 11 : 646256 The Valproate Mediates Radio-Bidirectional Regulation Through RFWD3-Dependent Ubiquitination on Rad51.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations