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ADAM17 antibody [HL1572]

Recombinant antibody
Anti-ADAM17 antibody [HL1572] used in Western Blot (WB). GTX637047
Anti-ADAM17 antibody [HL1572] used in Western Blot (WB). GTX637047
Anti-ADAM17 antibody [HL1572] used in IHC (Paraffin sections) (IHC-P). GTX637047
Anti-ADAM17 antibody [HL1572] used in IHC (Paraffin sections) (IHC-P). GTX637047
Anti-ADAM17 antibody [HL1572] used in IHC (Paraffin sections) (IHC-P). GTX637047

Cat. No. GTX637047

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1572

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all ADAM17 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Product Note

This antibody was raised against human ADAM17 Intracellular domain.

Predict Reactivity

Rat, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human ADAM17. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADAM metallopeptidase domain 17 , ADAM18 , CD156B , CSVP , NISBD , NISBD1 , TACE

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands. The encoded protein also plays a prominent role in the activation of the Notch signaling pathway. Elevated expression of this gene has been observed in specific cell types derived from psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn's disease patients, suggesting that the encoded protein may play a role in autoimmune disease. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-ADAM17 antibody [HL1572] used in Western Blot (WB). GTX637047

GTX637047 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [HL1572] (GTX637047) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ADAM17 antibody [HL1572] used in Western Blot (WB). GTX637047

GTX637047 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [HL1572] (GTX637047) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ADAM17 antibody [HL1572] used in IHC (Paraffin sections) (IHC-P). GTX637047

GTX637047 IHC-P Image

ADAM17 antibody [HL1572] detects ADAM17 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ADAM17 stained by ADAM17 antibody [HL1572] (GTX637047) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ADAM17 antibody [HL1572] used in IHC (Paraffin sections) (IHC-P). GTX637047

GTX637047 IHC-P Image

ADAM17 antibody [HL1572] detects ADAM17 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat mammary gland.
ADAM17 stained by ADAM17 antibody [HL1572] (GTX637047) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ADAM17 antibody [HL1572] used in IHC (Paraffin sections) (IHC-P). GTX637047

GTX637047 IHC-P Image

ADAM17 antibody [HL1572] detects ADAM17 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog tissues.
ADAM17 stained by ADAM17 antibody [HL1572] (GTX637047) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations