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ATM antibody

KO/KD Orthogonal
Anti-ATM antibody used in Western Blot (WB). GTX111106
Anti-ATM antibody used in IHC (Paraffin sections) (IHC-P). GTX111106
Anti-ATM antibody used in Western Blot (WB). GTX111106
Anti-ATM antibody used in Western Blot (WB). GTX111106
Anti-ATM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111106
Anti-ATM antibody used in Western Blot (WB). GTX111106

Cat. No. GTX111106

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Cat
Package
100 μl ($399),
25 μl ($169)
See all ATM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

351 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176288

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

Cellular Localization

Nucleus , Cytoplasmic vesicle

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATM antibody used in Western Blot (WB). GTX111106

GTX111106 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (GTX111106) diluted at 1:500.

Anti-ATM antibody used in IHC (Paraffin sections) (IHC-P). GTX111106

GTX111106 IHC-P Image

ATM antibody detects ATM protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat spleen.
ATM stained by ATM antibody (GTX111106) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATM antibody used in Western Blot (WB). GTX111106

GTX111106 WB Image

PG-4 whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (GTX111106) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ATM antibody used in Western Blot (WB). GTX111106

GTX111106 WB Image

Orthogonal

ATM antibody detects ATM protein by western blot analysis. whole cell extracts and extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (GTX111106) at a dilution of 1:500.

Anti-ATM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111106

GTX111106 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATM(GTX111106) antibody at 1:500 dilution.

Anti-ATM antibody used in Western Blot (WB). GTX111106

GTX111106 WB Image

KO/KD

The data was published in the journal Nucleic Acids Res in 2019. PMID: 31114877

REFERENCE

Application Reference

Velichko AK et al. Nucleic Acids Res 2019; (Epub) Hypoosmotic stress induces R loop formation in nucleoli and ATR/ATM-dependent silencing of nucleolar transcription.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169