Adenosine A1 Receptor antibody [HL2442]

Name: Adenosine A1 Receptor antibody [HL2442]
Brand: GeneTex
SKU: GTX638758
Price: 399.00 USD

Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758

Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758

Cat. No. GTX638758

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2442
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat, Dog
Package	100 μl ($399), 25 μl ($169)

See all Adenosine A1 Receptor products

Datasheet

View product citations for antibody GTX638758 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Product Note

This antibody was raised against human Adenosine A1 Receptor Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Adenosine A1 Receptor. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine A1 receptor , RDC7

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 134 ADORA1
UniProt: P30542 ADORA1

Research Area

DATA IMAGES

GTX638758 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638758 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638758 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX638758 WB Image

Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:2000 and the highly cited competitor's diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX638758 IHC-P Image

Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX638758 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX638758 WB Image

SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638758 IHC-P Image

Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX638758 IHC-P Image

Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX638758 WB Image

Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638758 IHC-P Image

Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty Custom Conjugations

Adenosine A1 Receptor antibody [HL2442]

Cat. No. GTX638758

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX638758 WB Image

GTX638758 WB Image

GTX638758 WB Image

GTX638758 WB Image

GTX638758 IHC-P Image

GTX638758 WB Image

GTX638758 WB Image

GTX638758 IHC-P Image

GTX638758 IHC-P Image

GTX638758 WB Image

GTX638758 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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