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CD133 antibody [HL1271]

Recombinant antibody Orthogonal Overexpression
Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673
Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673
Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673
Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673

Cat. No. GTX636673

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1271

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CD133 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Product Note

This antibody was raised against human CD133 Extracellular domain.

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD133. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910041

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prominin 1 , AC133 , CD133 , CORD12 , MCDR2 , MSTP061 , PROML1 , RP41 , STGD4

Cellular Localization

Apical cell membrane,Endoplasmic reticulum

Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673

GTX636673 IHC-P Image

CD133 antibody [HL1271] detects CD133 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
CD133 stained by CD133 antibody [HL1271] (GTX636673) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673

GTX636673 IHC-P Image

CD133 antibody [HL1271] detects CD133 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
CD133 stained by CD133 antibody [HL1271] (GTX636673) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673

GTX636673 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (GTX636673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673

GTX636673 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (GTX636673) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations