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CD16 + CD32 antibody [2.4G2]

Cat. No. GTX01453

Reference 283

Host

 Rat

Clonality

 Monoclonal

Clone Name

 2.4G2

Isotype

 IgG2b

Application

 FACS, IP, Neutralizing/Inhibition

Reactivity

 Mouse
Package
100 μg ($119)
See all CD16 + CD32 products
PRODUCT

Summary

The 2.4G2 antibody is specific for a common epitope found in the extracellular regions of mouse Fc-receptors Fc-gamma II (CD32) and Fc-gamma III (CD16). As these are receptors for the Fc portion of mouse IgG, they may also bind laboratory antibody preparations and products used in a variety of cell analysis protocols such as flow cytometry, immunohistochemistry and functional cell assays. The 2.4G2 antibody is therefore widely used as a pre-treatment reagent to block binding of specific antibodies of interest, e.g. fluorescently conjugated antibodies, to Fc receptors via their Fc domains and contributing to “non-specific” staining.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
IP Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Purity

> 90% (determined by SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fc receptor, IgG, low affinity IIb , Fc receptor, IgG, low affinity III , AI528646 , CD32 , F630109E10Rik , Fc[g]RII , FcgRII , Fcgr2 , Fcgr2a , Fcr-2 , Fcr-3 , Ly-17 , Ly-m20 , LyM-1 , Lym-1 , fcRII , CD16

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Database

Research Area

REFERENCE

Clone Reference

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Kallis MP et al. J Transl Med 2020; 18 (1):183 Pharmacological prevention of surgery-accelerated metastasis in an animal model of osteosarcoma.
Becerril-Garc?a MA et al. Front Immunol 2020; 11:744 Langerhans Cells From Mice at Birth Express Endocytic- and Pattern Recognition-Receptors, Migrate to Draining Lymph Nodes Ferrying Antigen and Activate Neonatal T Cells in vivo.
Patta I et al. Nucleic Acids Res 2020; 48 (11):5873-5890 Dynamic regulation of chromatin organizer SATB1 via TCR-induced alternative promoter switch during T-cell development.
Wilhelmson AS et al. Front Immunol 2020; 11:1342 Androgen Receptors in Epithelial Cells Regulate Thymopoiesis and Recent Thymic Emigrants in Male Mice.
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Clone Reference
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2012; CD86 is expressed on murine hematopoietic stem cells and denotes lymphopoietic potential.
Application : Neutralizing/Inhibition
Reactivity : Mouse
2012; The development of colitogenic CD4(+) T cells is regulated by IL-7 in collaboration with NK cell function in a murine model of colitis.
Application : Neutralizing/Inhibition
Reactivity : Mouse
2012; Subcellular distribution of Lck during CD4 T-cell maturation in the thymic medulla regulates the T-cell activation threshold.
Application : Neutralizing/Inhibition
Reactivity : Mouse
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Stoeker L et al. Clin Vaccine Immunol 2011; 18 (11):1834-44 Assessment of Lactobacillus gasseri as a candidate oral vaccine vector.
Application : Neutralizing/Inhibition
Reactivity : Mouse
Butler NS et al. Nat Immunol 2011; 13 (2):188-95 Therapeutic blockade of PD-L1 and LAG-3 rapidly clears established blood-stage Plasmodium infection.
2011; IL-2 contributes to maintaining a balance between CD4+Foxp3+ regulatory T cells and effector CD4+ T cells required for immune control of blood-stage malaria infection.
Application : Neutralizing/Inhibition
Reactivity : Mouse
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Oreshkova T et al. Immunology 2009; 128 (2):271-86 Beta(2) integrin deficiency yields unconventional double-negative T cells distinct from mature classical natural killer T cells in mice.
Amano S et al. J Cell Physiol 2009; 221 (1):40-53 A novel osteoclast precursor cell line, 4B12, recapitulates the features of primary osteoclast differentiation and function: enhanced transfection efficiency before and after differentiation.
Rotolo JA et al. Blood 2009; 114 (17):3693-706 Cytolytic T cells induce ceramide-rich platforms in target cell membranes to initiate graft-versus-host disease.
Park H et al. Mol Biol Cell 2009; 20 (21):4500-8 Cdc42 regulates Fc gamma receptor-mediated phagocytosis through the activation and phosphorylation of Wiskott-Aldrich syndrome protein (WASP) and neural-WASP.
Smith E et al. J Immunol 2009; 183 (9):5685-93 T-lineage cells require the thymus but not VDJ recombination to produce IL-17A and regulate granulopoiesis in vivo.
Manicone AM et al. Am J Pathol 2009; 175 (6):2319-31 Matrilysin (Matrix Metalloproteinase-7) regulates anti-inflammatory and antifibrotic pulmonary dendritic cells that express CD103 (alpha(E)beta(7)-integrin).
Gulani J et al. Comp Med 2009; 59 (6):534-44 The effect of Helicobacter hepaticus infection on immune responses specific to herpes simplex virus type 1 and characteristics of dendritic cells.
2008; Sustained NKG2D engagement induces cross-tolerance of multiple distinct NK cell activation pathways.
Application : IP, Neutralizing/Inhibition
Reactivity : Mouse
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