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CD81 antibody [HL1666]

Recombinant antibody Overexpression
Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264
Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264
Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264
Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264
Anti-CD81 antibody [HL1666] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637264
Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264

Cat. No. GTX637264

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1666

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CD81 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Product Note

This antibody was raised against human CD81 Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD81. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD81 molecule , CVID6 , S5.7 , TAPA1 , TSPAN28

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES

Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264

GTX637264 WB Image

SU86.86 exsosome extracts (1 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody [HL1666] (GTX637264) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264

GTX637264 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody [HL1666] (GTX637264) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264

GTX637264 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody [HL1666] (GTX637264) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264

GTX637264 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody [HL1666] (GTX637264) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD81 antibody [HL1666] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637264

GTX637264 ICC/IF Image

CD81 antibody [HL1666] detects CD81 protein by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD81 stained by CD81 antibody [HL1666] (GTX637264) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264

GTX637264 WB Image

Jurkat membrane extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody [HL1666] (GTX637264) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations