APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against human CXCR4 Intracellular domain.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human CXCR4. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C-X-C motif chemokine receptor 4 , CD184 , D2S201E , FB22 , HM89 , HSY3RR , LAP-3 , LAP3 , LCR1 , LESTR , NPY3R , NPYR , NPYRL , NPYY3R , WHIM , WHIMS
Cellular Localization
Cell membrane,Cell junction,Early endosome,Lysosome
Background
This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX638646 WB Image
Non-transfected (–) and transfected (+) unboiled Jurkat whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2424] (GTX638646) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638646 WB Image
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR4 antibody [HL2424] (GTX638646) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638646 ICC/IF Image
CXCR4 antibody [HL2424] detects CXCR4 protein at cell membrane by immunofluorescent analysis.Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CXCR4 stained by CXCR4 antibody [HL2424] (GTX638646) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638646 IHC-P Image
CXCR4 antibody [HL2424] detects CXCR4 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human pancreatic cancer.CXCR4 stained by CXCR4 antibody [HL2424] (GTX638646) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638646 IHC-P Image
CXCR4 antibody [HL2424] detects CXCR4 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse salivary gland tissues.CXCR4 stained by CXCR4 antibody [HL2424] (GTX638646) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for CXCR4 antibody [HL2424] (GTX638646). Be the first to share your publications with this product.
REVIEW
CXCR4 antibody [HL2424]
Cat. No. GTX638646
Rating
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( Average 5 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 20-Jul-2023
Application Tested :
WB
Sample Species :
Hu
Sample :
boiled and unboiled Jurkat whole cell lysate and membrance extracts
Amount used :
30μg
Blocking :
5% non-fat milk in TBST, 25°C, 0.5-1Hr
Primary Antibody :
1:1000, 4°C, >12Hr
Lane Description :
WCL : Whole Cell Lysate
ME : Membrance Extracts