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Chikungunya virus nsP4 antibody [HL2582]

Recombinant antibody
Anti-Chikungunya virus nsP4 antibody [HL2582] used in Western Blot (WB). GTX638960
Anti-Chikungunya virus nsP4 antibody [HL2582] used in Western Blot (WB). GTX638960
Anti-Chikungunya virus nsP4 antibody [HL2582] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638960
Anti-Chikungunya virus nsP4 antibody [HL2582] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638960

Cat. No. GTX638960

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2582

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Chikungunya Virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chikungunya Virus

Immunogen

Recombinant fragment of Chikungunya virus nsP4 (strain S27-African prototype)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Chikungunya virus nsP4 antibody [HL2582] used in Western Blot (WB). GTX638960

GTX638960 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chikungunya virus nsP4 antibody [HL2582] (GTX638960) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Chikungunya virus nsP4 antibody [HL2582] used in Western Blot (WB). GTX638960

GTX638960 WB Image

Chikungunya viral lysate (0.1 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Chikungunya virus nsP4 antibody [HL2582] (GTX638960) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-Chikungunya virus nsP4 antibody [HL2582] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638960

GTX638960 ICC/IF Image

Chikungunya virus nsP4 antibody [HL2582] detects Chikungunya virus nsP4 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Chikungunya virus nsP4 stained by Chikungunya virus nsP4 antibody [HL2582] (GTX638960) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Chikungunya virus nsP4 antibody [HL2582] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638960

GTX638960 ICC/IF Image

Immunofluorescent analysis of Chikungunya virus infected cells using Chikungunya virus nsP4 antibody [HL2582] antibody (GTX638960).
Sample: Chikungunya virus infected cells slide.
Green: Chikungunya virus nsP4 antibody [HL2582] antibody (GTX638960) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations