Claudin 3 antibody [HL1527]

Cat. No. GTX637000

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1527
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all Claudin 3 products

Datasheet

View product citations for antibody GTX637000 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Product Note

This antibody was raised against human Claudin 3 Intracellular domain.

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Claudin 3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

claudin 3 , C7orf1 , CPE-R2 , CPETR2 , HRVP1 , RVP1

Cellular Localization

Cell membrane

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]

Database

Gene ID: 1365 CLDN3
UniProt: O15551 CLDN3

Research Area

DATA IMAGES

GTX637000 ICC/IF Image

Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 and HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Claudin 3 stained by Claudin 3 antibody [HL1527] (GTX637000) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX637000 IHC-P Image

Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
Claudin 3 stained by Claudin 3 antibody [HL1527] (GTX637000) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637000 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 3 antibody [HL1527] (GTX637000) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations