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DDDDK tag antibody (Biotin)

Anti-DDDDK tag antibody (Biotin) used in ELISA (ELISA). GTX77459

Cat. No. GTX77459

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, IHC

Reactivity

 Species independent
Package
100 μg ($399)
See all DDDDK tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1 - 0.01 ug/ml
ELISA 0.1 - 0.01 ug/ml
IHC 2.0 - 0.2 ug/ml
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, stabilizer

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Rabbits were immunized with the xxxDDDDK peptide conjugated with KLH. After multiple immunizations in Freunds adjuvant, serum was collected. Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase then conjugated with biotin

Purification

Purified by antigen-affinity chromatography

Conjugation

Biotin

RRID

AB_379970

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-DDDDK tag antibody (Biotin) used in ELISA (ELISA). GTX77459

GTX77459 ELISA Image

ELISA analysis of immunogen using GTX77459 DDDDK tag antibody (Biotin).

REFERENCE

Application Reference

Ode T et al. Exp Cell Res 2016; (Epub) PDMP, a ceramide analogue, acts as an inhibitor of mTORC1 by inducing its translocation from lysosome to endoplasmic reticulum.
Application : WB
Terasawa K et al. J Immunol Methods 2015; 420 : 43110 Preferential recognition of isocitrate dehydrogenase by a rabbit monoclonal antibody (ab124797) against the C-terminal peptide of RANKL.
Application : WB
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399