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E-Cadherin antibody [HL1229]

Recombinant antibody KO/KD Orthogonal
Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577
Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577
Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577
Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577
Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577
Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577
Anti-E-Cadherin antibody [HL1229] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636577
Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577
Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577
Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577
Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577

Cat. No. GTX636577

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1229

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all E-Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910011

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

Cellular Localization

Cell junction,Cell membrane,Endosome

Background

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577

GTX636577 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577

GTX636577 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577

GTX636577 IHC-P Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577

GTX636577 IHC-P Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577

GTX636577 IHC-P Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577

GTX636577 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody [HL1229] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636577

GTX636577 ICC/IF Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577

GTX636577 IHC-P Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog mammary gland.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577

GTX636577 IHC-P Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein by immunohistochemical analysis.
Sample: Paraffin-embedded cat pancreas.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [HL1229] used in IHC (Paraffin sections) (IHC-P). GTX636577

GTX636577 IHC-P Image

E-Cadherin antibody [HL1229] detects E-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
E-Cadherin stained by E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody [HL1229] used in Western Blot (WB). GTX636577

GTX636577 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (GTX636577) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Pai JT et al. Int J Mol Sci 2022; 23 (3) Propolin G-Suppressed Epithelial-to-Mesenchymal Transition in Triple-Negative Breast Cancer Cells via Glycogen Synthase Kinase 3β-Mediated Snail and HDAC6-Regulated Vimentin Degradation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations