GeneTex

  • Country / Location Selection

United States (US)

EIF5A antibody

Anti-EIF5A antibody used in Western Blot (WB). GTX111013
Anti-EIF5A antibody used in Western Blot (WB). GTX111013
Anti-EIF5A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111013
Anti-EIF5A antibody used in IHC (Paraffin sections) (IHC-P). GTX111013
Anti-EIF5A antibody used in Western Blot (WB). GTX111013

Cat. No. GTX111013

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EIF5A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

PC-12 , Rat2

Predict Reactivity

Zebrafish, Sheep, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human EIF5A Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950191

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 5A , EIF-5A , EIF5A1 , eIF5AI

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , nuclear pore complex

Background

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of TP53/p53 and TP53/p53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.

Database

Research Area

DATA IMAGES

Anti-EIF5A antibody used in Western Blot (WB). GTX111013

GTX111013 WB Image

EIF5A antibody detects EIF5A protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
EIF5A antibody (GTX111013) dilution: 1:5000

Anti-EIF5A antibody used in Western Blot (WB). GTX111013

GTX111013 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with EIF5A antibody (GTX111013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EIF5A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111013

GTX111013 ICC/IF Image

EIF5A antibody detects EIF5A protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF5A stained by EIF5A antibody (GTX111013) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EIF5A antibody used in IHC (Paraffin sections) (IHC-P). GTX111013

GTX111013 IHC-P Image

EIF5A antibody detects EIF5A protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EIF5A antibody (GTX111013) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF5A antibody used in Western Blot (WB). GTX111013

GTX111013 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with EIF5A antibody (GTX111013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Martinez-Rocha AL et al. Sci Rep 2016; 6 : 24698 Posttranslational hypusination of the eukaryotic translation initiation factor-5A regulates Fusarium graminearum virulence.
Application : WB
Submit a Reference

REVIEW

There are currently no reviews for EIF5A antibody (GTX111013). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169