FACL4 antibody [C3], C-term

Cat. No. GTX100260

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all FACL4 products

View product citations for antibody GTX100260 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:1000-1:5000

Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FACL4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240790

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA synthetase long chain family member 4 , ACS4 , FACL4 , LACS4 , MRX63 , MRX68

Cellular Localization

Mitochondrion outer membrane; Single-pass type III membrane protein , Peroxisome membrane; Single-pass type III membrane protein , Microsome membrane; Single-pass type III membrane protein , Endoplasmic reticulum membrane; Single-pass type III membrane pr

Background

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Database

Gene ID: 2182 ACSL4
UniProt: O60488 ACSL4

Research Area

DATA IMAGES

GTX100260 ICC/IF Image

FACL4 antibody [C3], C-term detects FACL4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FACL4 stained by FACL4 antibody [C3], C-term (GTX100260) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX100260 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term (GTX100260) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100260 WB Image

GTX100260 IHC-P Image

FACL4 antibody [C3], C-term detects FACL4 protein at cytosol on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
FACL4 antibody [C3], C-term (GTX100260) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100260 IHC-P Image

FACL4 antibody [C3], C-term detects FACL4 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
FACL4 stained by FACL4 antibody [C3], C-term (GTX100260) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100260 WB Image

GTX100260 IHC-P Image

FACL4 antibody [C3], C-term detects FACL4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
FACL4 stained by FACL4 antibody [C3], C-term (GTX100260) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100260 IHC-P Image

FACL4 antibody [C3], C-term detects FACL4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
FACL4 stained by FACL4 antibody [C3], C-term (GTX100260) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100260 IHC-P Image

FACL4 antibody [C3], C-term detects FACL4 protein at cytosol on mouse lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
FACL4 antibody [C3], C-term (GTX100260) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100260 IP Image

Immunoprecipitation of FACL4 protein from HeLa whole cell extracts using 5 μg of FACL4 antibody [C3], C-term (GTX100260).
Western blot analysis was performed using FACL4 antibody [C3], C-term (GTX100260) diluted at 1:500.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.