Galectin 3 antibody [HL2020]

Name: Galectin 3 antibody [HL2020]
Brand: GeneTex
SKU: GTX637921
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Galectin 3 antibody [HL2020] used in Western Blot (WB). GTX637921

Anti-Galectin 3 antibody [HL2020] used in IHC (Paraffin sections) (IHC-P). GTX637921

Anti-Galectin 3 antibody [HL2020] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637921

Cat. No. GTX637921

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2020
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Cat, Dog
Package	100 μl ($399), 25 μl ($169)

See all Galectin 3 products

Datasheet

View product citations for antibody GTX637921 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Galectin 3

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

Cellular Localization

Cytoplasm,Nucleus,Secreted

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Database

Gene ID: 3958 LGALS3
UniProt: P17931 LGALS3

Research Area

DATA IMAGES

GTX637921 WB Image

Wild-type (WT) and LGALS3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637921 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX637921 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637921 IHC-P Image

Galectin 3 antibody [HL2020] detects Galectin 3 protein at cell membrane and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat stomach.
Galectin 3 stained by Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637921 IHC-P Image

Galectin 3 antibody [HL2020] detects Galectin 3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
Galectin 3 stained by Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637921 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637921 ICC/IF Image

Galectin 3 antibody [HL2020] detects Galectin 3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Galectin 3 stained by Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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