Goat Anti-Rabbit IgG antibody (DyLight488)

Cat. No. GTX213110-04

Reference 18

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, FACS
Reactivity	Rabbit
Package	500 μl ($149)

See all Rabbit IgG products

View product citations for antibody GTX213110-04 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	1:100-1:2000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IHC-Wm	1:100-1:500
FACS	1:50-1:1000

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Highly purified whole rabbit IgG

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography.

Conjugation

DyLight488

RRID

AB_2887579

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Goat Anti Rabbit IgG

DATA IMAGES

GTX213110-04 IHC-P Image

Double-labeled immunofluorescence photomicrographs of paraffin-embedded sections of mouse colon.
Green: E-Cadherin antibody (GTX100443) diluted at 1:500. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX213110-04 IHC-Fr Image

Double-labeled immunofluorescence photomicrographs of frozen sections of mouse brain.
Green: Vimentin antibody (GTX100619) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Red: ME1 antibody [GT15611] (GTX632190) diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).
Blue: Nuclear staining with Hoechst 33342.

GTX213110-04 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
The signal was developed using Goat Anti-Rabbit IgG antibody (DyLight488) (GTX213110-04) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX213110-04 FACS Image

CD81 antibody (GTX101766) detects CD81 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: CD81 antibody (GTX101766) dilution: 1:50.
The Rabbit IgG antibody (DyLight488) (GTX213110-04) was used to detect the primary antibody.

GTX213110-04 FACS Image

Glypican 1 antibody [N3C3] (GTX104557) detects Glypican 1 protein by flow cytometry analysis.
Sample: A431 cell.
Black: Unlabelled sample was used as a control.
Red: Glypican 1 antibody [N3C3] (GTX104557) dilution: 1:50.
Acquisition of 20,000 events were collected using the Rabbit IgG (DyLight488) (GTX113110-04) secondary antibody for FACS analysis.

GTX213110-04 IHC-Wm Image

Whole mount immunohistochemical analysis of paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo using Pax2a antibody (GTX128127) detected by anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
GTX128127 diluted at 1:100 and incubated overnight at 4ºC.
GTX213110-04 diluted at 1:500 and incubated 3 hours at room temperature.

REFERENCE

Application Reference

Hung YH et al. J Pers Med 2024; 14 (3) Chromatin Remodeling-Related PRDM1 Increases Stomach Cancer Proliferation and Is Counteracted by Bromodomain Inhibitor.

YH Hung et al. J Pers Med 2024; 14 (3) : 224 Chromatin Remodeling-Related PRDM1 Increases Stomach Cancer Proliferation and Is Counteracted by Bromodomain Inhibitor

Application : ICC/IF

SH Chen et al. Neurotherapeutics 2024; 21 (3) : e00336 Ultrahigh frequency transcutaneous electrical nerve stimulation for neuropathic pain alleviation and neuromodulation

Application : IHC-Fr

SH Yeh et al. Cell Transplant 2024; 33 : 9.6369E+15 Proliferation and Differentiation Potential of Bone Marrow–Derived Mesenchymal Stem Cells From Children With Polydactyly and Adults With Basal Joint Arthritis

Hsia Y et al. Int J Nanomedicine 2023; 18 : 4253-4274 A Dual Concentration-Tailored Cytokine-Chemo Nanosystem to Alleviate Multidrug Resistance and Redirect Balance of Cancer Proliferation and Apoptosis.

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REVIEW

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Application Reference

Hung YH et al. J Pers Med 2024; 14 (3) Chromatin Remodeling-Related PRDM1 Increases Stomach Cancer Proliferation and Is Counteracted by Bromodomain Inhibitor.

YH Hung et al. J Pers Med 2024; 14 (3):224 Chromatin Remodeling-Related PRDM1 Increases Stomach Cancer Proliferation and Is Counteracted by Bromodomain Inhibitor

Application : ICC/IF

SH Chen et al. Neurotherapeutics 2024; 21 (3):e00336 Ultrahigh frequency transcutaneous electrical nerve stimulation for neuropathic pain alleviation and neuromodulation

Application : IHC-Fr

SH Yeh et al. Cell Transplant 2024; 33:9.6369E+15 Proliferation and Differentiation Potential of Bone Marrow–Derived Mesenchymal Stem Cells From Children With Polydactyly and Adults With Basal Joint Arthritis

Hsia Y et al. Int J Nanomedicine 2023; 18:4253-4274 A Dual Concentration-Tailored Cytokine-Chemo Nanosystem to Alleviate Multidrug Resistance and Redirect Balance of Cancer Proliferation and Apoptosis.

Hoai-Nga Thi Nguyen et al. Front Oncol 2022; 12:923467 SARS-CoV-2 M Protein Facilitates Malignant Transformation of Breast Cancer Cells.

Tangeda V et al. Cell Death Dis 2022; 13 (3):241

Yen-Chen Chen et al. Pharmaceuticals (Basel) 2021; 14 (4) Micafungin Inhibits Dengue Virus Infection through the Disruption of Virus Binding, Entry, and Stability.