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HA tag antibody (HRP)

Anti-HA tag antibody (HRP) used in Western Blot (WB). GTX77400

Cat. No. GTX77400

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Species independent
Package
100 μl ($399)
See all HA tag products

APPLICATION

Application Note

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ELISA. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Positive Control

HA-NRAS-tranfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Rabbits were immunized with HA-tag conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support then conjugated with HRP ( Horseradish Peroxidase).

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_379994

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-HA tag antibody (HRP) used in Western Blot (WB). GTX77400

GTX77400 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HA Tag antibody (HRP) (GTX77400) diluted at 1:3000.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 399