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HEY1 antibody [HL2367]

Recombinant antibody Overexpression
Anti-HEY1 antibody [HL2367] used in Western Blot (WB). GTX638573
Anti-HEY1 antibody [HL2367] used in Western Blot (WB). GTX638573
Anti-HEY1 antibody [HL2367] used in Western Blot (WB). GTX638573
Anti-HEY1 antibody [HL2367] used in Western Blot (WB). GTX638573
Anti-HEY1 antibody [HL2367] used in Western Blot (WB). GTX638573
Anti-HEY1 antibody [HL2367] used in IHC (Paraffin sections) (IHC-P). GTX638573

Cat. No. GTX638573

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2367

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Dog
Package
100 μl ($399),
25 μl ($169)
See all HEY1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human HEY1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hes related family bHLH transcription factor with YRPW motif 1 , BHLHb31 , CHF2 , HERP2 , HESR1 , HRT-1 , NERP2 , OAF1 , hHRT1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HEY1 antibody [HL2367] used in Western Blot (WB). GTX638573

GTX638573 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HEY1 antibody [HL2367] used in Western Blot (WB). GTX638573

GTX638573 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HEY1 antibody [HL2367] used in Western Blot (WB). GTX638573

GTX638573 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HEY1 antibody [HL2367] used in Western Blot (WB). GTX638573

GTX638573 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-HEY1 antibody [HL2367] used in Western Blot (WB). GTX638573

GTX638573 WB Image

Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HEY1 antibody [HL2367] used in IHC (Paraffin sections) (IHC-P). GTX638573

GTX638573 IHC-P Image

HEY1 antibody [HL2367] detects HEY1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
HEY1 stained by HEY1 antibody [HL2367] (GTX638573) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169