Summary
G-CSF (Granulocyte-colony stimulating factor/CSF3) is the major growth factor driving the generation of mature granulocytes from committed bone marrow myeloid progenitors. This biologically active recombinant human G-CSF is an 18.7 kDa globular protein formed by a 174-amino acid polypeptide with two intramolecular disulfide bonds. Human and murine G-CSFs are highly similar and show reciprocal biological cross-reactivity.
Form
Lyophilized powder
Buffer
Reconstitute with ddH₂O to 0.1 mg/ml. Lyophilized from 50mM PBS (pH7.4), 200mM NaCl, 5% Trehalose.
Preservative
No preservative
Storage
Store at 4ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.
Region/Sequence
Human full-length G-CSF protein (31-204 a.a. of NP_757373.1, signal peptide removed)
Expression System
E. Coli
Purity
>98%
Endotoxin
< 1 EU/ug
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
colony stimulating factor 3 , C17orf33 , CSF3OS , GCSF
Cellular Localization
Secreted
Background
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Database
Research Area