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ISG15 antibody [HL2017]

Recombinant antibody KO/KD Orthogonal
Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918
Anti-ISG15 antibody [HL2017] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637918
Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918
Anti-ISG15 antibody [HL2017] used in IHC (Paraffin sections) (IHC-P). GTX637918
Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918
Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918

Cat. No. GTX637918

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2017

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Cat
Package
100 μl ($399),
25 μl ($169)
See all ISG15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human ISG15

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ISG15 ubiquitin like modifier , G1P2 , IFI15 , IMD38 , IP17 , UCRP , hUCRP

Cellular Localization

Cytoplasm,Secreted

Background

The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]

Database

Research Area

DATA IMAGES

Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918

GTX637918 WB Image

KO/KD

Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (GTX637918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ISG15 antibody [HL2017] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637918

GTX637918 ICC/IF Image

ISG15 antibody [HL2017] detects ISG15 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: ISG15 stained by ISG15 antibody [HL2017] (GTX637918) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918

GTX637918 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (GTX637918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ISG15 antibody [HL2017] used in IHC (Paraffin sections) (IHC-P). GTX637918

GTX637918 IHC-P Image

ISG15 antibody [HL2017] detects ISG15 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat brain.
ISG15 stained by ISG15 antibody [HL2017] (GTX637918) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918

GTX637918 WB Image

Orthogonal

Untreated (–) and treated (+) A549 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (GTX637918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ISG15 antibody [HL2017] used in Western Blot (WB). GTX637918

GTX637918 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (GTX637918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations