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LC3B antibody [GT1187]

Recombinant antibody KO/KD Orthogonal
Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949
Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949
Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949
Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949
Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949
Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949
Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949
Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949
Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949

Cat. No. GTX00949

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1187

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all LC3B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Observed MW

14, 16 kDa. The observed M.W. is based on the following publication. PMID: 31576176

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human LC3B.

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B , MAP1LC3Ba , APG8b

Cellular Localization

Cytoplasm,Cytoskeleton,Endomembrane system,Cytoplasmic vesicle,Autophagosome membrane,Autophagosome

Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949

GTX00949 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT1187] (GTX00949) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949

GTX00949 ICC/IF Image

Orthogonal

ICC/IF analysis of PFA-fixed HeLa cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated HeLa cells
Left : HeLa cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949

GTX00949 ICC/IF Image

Orthogonal

ICC/IF analysis of PFA-fixed NIH-3T3 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated NIH-3T3 cells
Left : NIH-3T3 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949

GTX00949 IHC-P Image

IHC-P analysis of Mouse spinal cord tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100

Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949

GTX00949 IHC-P Image

Orthogonal

IHC-P analysis of Rat brain tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100

Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949

GTX00949 IHC-P Image

IHC-P analysis of Human brain tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100

Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949

GTX00949 ICC/IF Image

ICC/IF analysis of PFA-fixed C6 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated C6 cells
Left : C6 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949

GTX00949 WB Image

KO/KD Orthogonal

WB analysis of C6 and NIH-3T3 cell extracts with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Dilution : 1:1000
Loading : 25μg

Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949

GTX00949 WB Image

KO/KD Orthogonal

WB analysis of wild-type (WT) and LC3B knockout (KO) 293T cell extracts with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399