Melanoma gp100 antibody [8A4]

Name: Melanoma gp100 antibody [8A4]
Brand: GeneTex
SKU: GTX83640
Price: 519.00 USD

Anti-Melanoma gp100 antibody [8A4] used in IHC (Paraffin sections) (IHC-P). GTX83640

Anti-Melanoma gp100 antibody [8A4] used in Western Blot (WB). GTX83640

Anti-Melanoma gp100 antibody [8A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83640

Cat. No. GTX83640

Host	Mouse
Clonality	Monoclonal
Clone Name	8A4
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Rat, Dog, Monkey
Package	100 μl ($519)

See all Melanoma gp100 products

Datasheet
Document

View product citations for antibody GTX83640 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	Assay dependent
IHC-P	1:150

Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human SILV expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

premelanosome protein , D12S53E , ME20 , ME20-M , ME20M , P1 , P100 , PMEL17 , SI , SIL , SILV , gp100

Cellular Localization

Golgi apparatus

Background

Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.

Database

Gene ID: 6490 PMEL
UniProt: P40967 PMEL

Research Area

DATA IMAGES

GTX83640 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83640 Melanoma gp100 antibody [8A4].

GTX83640 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83640 Melanoma gp100 antibody [8A4].

GTX83640 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX83640 Melanoma gp100 antibody [8A4].

GTX83640 WB Image

WB analysis of HEK293T cells transfected with Melanoma gp100 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83640 Melanoma gp100 antibody [8A4].
Loading : 5 ug per lane

GTX83640 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX83640 Melanoma gp100 antibody [8A4].

GTX83640 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX83640 Melanoma gp100 antibody [8A4].

GTX83640 WB Image

WB analysis of various cell lines using GTX83640 Melanoma gp100 antibody [8A4].
Loading : 35 ug per lane

GTX83640 IHC-P Image

IHC-P analysis of human prostate tissue using GTX83640 Melanoma gp100 antibody [8A4].

GTX83640 ICC/IF Image

ICC/IF analysis of A549 cells using GTX83640 Melanoma gp100 antibody [8A4].

GTX83640 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX83640 Melanoma gp100 antibody [8A4].

GTX83640 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Melanoma gp100 plasmid using GTX83640 Melanoma gp100 antibody [8A4].

GTX83640 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX83640 Melanoma gp100 antibody [8A4].

GTX83640 IHC-P Image

IHC-P analysis of human liver tissue using GTX83640 Melanoma gp100 antibody [8A4].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

Melanoma gp100 antibody [8A4]

Cat. No. GTX83640

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX83640 IHC-P Image

GTX83640 IHC-P Image

GTX83640 IHC-P Image

GTX83640 WB Image

GTX83640 IHC-P Image

GTX83640 IHC-P Image

GTX83640 WB Image

GTX83640 IHC-P Image

GTX83640 ICC/IF Image

GTX83640 IHC-P Image

GTX83640 ICC/IF Image

GTX83640 IHC-P Image

GTX83640 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.