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Occludin antibody

Orthogonal
Anti-Occludin antibody used in Western Blot (WB). GTX114949
Anti-Occludin antibody used in IHC (Frozen sections) (IHC-Fr). GTX114949
Anti-Occludin antibody used in IHC (Paraffin sections) (IHC-P). GTX114949
Anti-Occludin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114949
Anti-Occludin antibody used in IHC (Paraffin sections) (IHC-P). GTX114949

Cat. No. GTX114949

Reference 18
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399),
25 μl ($169)
See all Occludin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Occludin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177242

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

occludin , BLCPMG , PPP1R115 , PTORCH1

Cellular Localization

Cell membrane

Background

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]

Database

Research Area

DATA IMAGES

Anti-Occludin antibody used in Western Blot (WB). GTX114949

GTX114949 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Occludin antibody (GTX114949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Occludin antibody used in IHC (Frozen sections) (IHC-Fr). GTX114949

GTX114949 IHC-Fr Image

Occludin antibody detects Occludin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Occludin protein stained by Occludin antibody (GTX114949) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Occludin antibody used in IHC (Paraffin sections) (IHC-P). GTX114949

GTX114949 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Occludin(GTX114949) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Occludin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114949

GTX114949 ICC/IF Image

Occludin antibody detects Occludin protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Occludin stained by Occludin antibody (GTX114949) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Occludin antibody used in IHC (Paraffin sections) (IHC-P). GTX114949

GTX114949 IHC-P Image

Occludin antibody detects Occludin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Occludin stained by Occludin antibody (GTX114949) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

D Gu et al. Immunobiology 2024; : 152796 Transcription suppression of GABARAP mediated by lncRNA XIST-EZH2 interaction triggers caspase-11-dependent inflammatory injury in ulcerative colitis
Application : WB
Kobayashi K et al. Tissue Barriers 2024; 12 (1) : 2184157 Yogurt starter strains ameliorate intestinal barrier dysfunction via activating AMPK in Caco-2 cells.
Xuehui Fan et al. Front Pharmacol 2022; 13 : 834948 S1PR3, as a Core Protein Related to Ischemic Stroke, is Involved in the Regulation of Blood-Brain Barrier Damage.
Walter Muranyi et al. iScience 2022; 25 (6) : 104383 Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type in?vitro model of the choroid plexus.
Hsiao-Chin Chou et al. J Biomed Sci 2022; 29 (1) : 40 DUSP3 regulates phosphorylation-mediated degradation of occludin and is required for maintaining epithelial tight junction.
Application : WB, ICC/IF
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

Occludin antibody Cat. No. GTX114949

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-Occludin antibody used in Western Blot (WB). GTX114949
Date : Anonymous submitted on 22-Jan-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human corneal epithelial cells with/without drug treatment
Amount used : 30μg
Blocking : commercial blocking buffer, RT°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 20Hr
Anti-Occludin antibody used in Western Blot (WB). GTX114949
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Brain Microvascular Endothelial Cells
Amount used : 30 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
D Gu et al. Immunobiology 2024;:152796 Transcription suppression of GABARAP mediated by lncRNA XIST-EZH2 interaction triggers caspase-11-dependent inflammatory injury in ulcerative colitis
Application : WB
Kobayashi K et al. Tissue Barriers 2024; 12 (1):2184157 Yogurt starter strains ameliorate intestinal barrier dysfunction via activating AMPK in Caco-2 cells.
Xuehui Fan et al. Front Pharmacol 2022; 13:834948 S1PR3, as a Core Protein Related to Ischemic Stroke, is Involved in the Regulation of Blood-Brain Barrier Damage.
Walter Muranyi et al. iScience 2022; 25 (6):104383 Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type in?vitro model of the choroid plexus.
Hsiao-Chin Chou et al. J Biomed Sci 2022; 29 (1):40 DUSP3 regulates phosphorylation-mediated degradation of occludin and is required for maintaining epithelial tight junction.
Application : WB, ICC/IF
Reactivity : Mouse
Cheng YQ et al. Neural Regen Res 2022; 17 (4):812-818 CircLphn3 protects the blood-brain barrier in traumatic brain injury.
Application : WB
Reactivity : Mouse
Wu C et al. Free Radic Biol Med 2021; 178:271-294 A novel mechanism linking ferroptosis and endoplasmic reticulum stress via the circPtpn14/miR-351-5p/5-LOX signaling in melatonin-mediated treatment of traumatic brain injury.
Reactivity : Mouse
Watanabe D et al. Pharmaceutics 2021; 13 (9) Characterization of a Primate Blood-Brain Barrier Co-Culture Model Prepared from Primary Brain Endothelial Cells, Pericytes and Astrocytes.
Application : WB
Reactivity : Monkey
Petrella C et al. Pharmacol Res 2021; 172:105795 Proneurogenic and neuroprotective effect of a multi strain probiotic mixture in a mouse model of acute inflammation: Involvement of the gut-brain axis.
Application : WB
Reactivity : Mouse
Li B et al. Food Funct 2021; 12 (12):5524-5538 Beneficial effects of Dendrobium officinale on metabolic hypertensive rats by triggering the enteric-origin SCFA-GPCR43/41 pathway.
Zhang LY et al. Evid Based Complement Alternat Med 2020; 2020:9017854 Guiqi Baizhu Decoction Alleviates Radiation Inflammation in Rats by Modulating the Composition of the Gut Microbiota.
Busatto S et al. J Nanobiotechnology 2020; 18 (1):162 Brain metastases-derived extracellular vesicles induce binding and aggregation of low-density lipoprotein.
Application : WB
Reactivity : Human
Alquisiras-Burgos I et al. Exp Neurol 2020; 330:113353 Resveratrol reduces cerebral edema through inhibition of de novo SUR1 expression induced after focal ischemia.
Application : IHC-Fr
Reactivity : Rat
Sun L et al. Mol Pharm 2020; 17 (3):757-768 Flash Fabrication of Orally Targeted Nanocomplexes for Improved Transport of Salmon Calcitonin across the Intestine.
Application : ICC/IF
Reactivity : Human
Tian H et al. Adv Healthc Mater 2018;:e1800285 uniform core-shell nanoparticles with thiolated hyaluronic acid coating to enhance oral delivery of *.
Reactivity : Human
Jo H et al. Food Chem Toxicol 2017; 108 (Pt A):203-213 Oxyresveratrol improves tight junction integrity through the PKC and MAPK signaling pathways in Caco-2 cells.
Application : WB
Reactivity : Human
He Z et al. Biomaterials 2017; 130:28-41 scalable fabrication of size-controlled chitosan nanoparticles for oral delivery of *.
Application : ICC/IF
Reactivity : Human
Hwang D et al. Biomed Pharmacother 2016; Conditioned medium from LS 174T goblet cells treated with oxyresveratrol strengthens tight junctions in Caco-2 cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169