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PHD1 antibody [GT1342]

Recombinant antibody
Anti-PHD1 antibody [GT1342] used in Western Blot (WB). GTX03254
Anti-PHD1 antibody [GT1342] used in Western Blot (WB). GTX03254
Anti-PHD1 antibody [GT1342] used in Western Blot (WB). GTX03254

Cat. No. GTX03254

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1342

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all PHD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PHD1/prolyl hydroxylase.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

egl-9 family hypoxia inducible factor 2 , EIT-6 , EIT6 , HIF-PH1 , HIFPH1 , HPH-1 , HPH-3 , PHD1

Cellular Localization

Nucleus

Background

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-PHD1 antibody [GT1342] used in Western Blot (WB). GTX03254

GTX03254 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PHD1 antibody [GT1342] (GTX03254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PHD1 antibody [GT1342] used in Western Blot (WB). GTX03254

GTX03254 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHD1 antibody [GT1342] (GTX03254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PHD1 antibody [GT1342] used in Western Blot (WB). GTX03254

GTX03254 WB Image

WB analysis of various samples using GTX03254 PHD1 antibody [GT1342].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 439