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Peregrin antibody [HL2064]

Recombinant antibody KO/KD Orthogonal Overexpression
Anti-Peregrin antibody [HL2064] used in Western Blot (WB). GTX637968
Anti-Peregrin antibody [HL2064] used in IHC (Paraffin sections) (IHC-P). GTX637968
Anti-Peregrin antibody [HL2064] used in Western Blot (WB). GTX637968
Anti-Peregrin antibody [HL2064] used in Western Blot (WB). GTX637968
Anti-Peregrin antibody [HL2064] used in Western Blot (WB). GTX637968

Cat. No. GTX637968

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2064

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Peregrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

137 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Peregrin

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bromodomain and PHD finger containing 1 , BR140 , IDDDFP

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a bromodomain, PHD finger and chromo/Tudor-related Pro-Trp-Trp-Pro (PWWP) domain containing protein. The encoded protein is a component of the MOZ/MORF histone acetyltransferase complexes which function as a transcriptional regulators. This protein binds to the catalytic MYST domains of the MOZ and MORF proteins and may play a role in stimulating acetyltransferase and transcriptional activity of the complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Peregrin antibody [HL2064] used in Western Blot (WB). GTX637968

GTX637968 WB Image

KO/KD

Wild-type (WT) and BRPF1 knockout (KO) 293T cell extracts (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [HL2064] (GTX637968) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-Peregrin antibody [HL2064] used in IHC (Paraffin sections) (IHC-P). GTX637968

GTX637968 IHC-P Image

Peregrin antibody [HL2064] detects Peregrin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Peregrin stained by Peregrin antibody [HL2064] (GTX637968) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Peregrin antibody [HL2064] used in Western Blot (WB). GTX637968

GTX637968 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [HL2064] (GTX637968) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Peregrin antibody [HL2064] used in Western Blot (WB). GTX637968

GTX637968 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [HL2064] (GTX637968) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Peregrin antibody [HL2064] used in Western Blot (WB). GTX637968

GTX637968 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [HL2064] (GTX637968) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations