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Goat Anti-Rat IgG antibody, F(ab')2 fragment, pre-adsorbed (FITC)

Goat Anti-Rat IgG antibody, F(ab')2 fragment, pre-adsorbed (FITC). GTX26115
Goat Anti-Rat IgG antibody, F(ab')2 fragment, pre-adsorbed (FITC). GTX26115

Cat. No. GTX26115

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG F(ab')2

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Rat
Package
250 μg ($179)
See all Rat IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:1000-1:5000
FACS 1:500-1:2500
ELISA 1:10000-1:50000
Not tested in other applications.

Product Note

Pre-adsorbed with Bovine, Horse, Human, Mouse, Rabbit and Sheep serum proteins. May react with immunoglobulins from other species.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat IgG whole molecule

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
From serum

Conjugation

Fluorescein isothiocyanate (FITC)
Ratio : 5.3 molecules FITC per Goat IgG F(ab')2 molecule.

RRID

AB_379337

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Goat Anti-Rat IgG antibody, F(ab')2 fragment, pre-adsorbed (FITC). GTX26115

GTX26115 WB Image

Western Blot of GTX26115 Sample: Rat IgG Load: 50 ng per lane Secondary antibody: GTX26115 at 1:1,000 for 60 min at RT Predicted/Observed size: 55 and 28 kDa.

Goat Anti-Rat IgG antibody, F(ab')2 fragment, pre-adsorbed (FITC). GTX26115

GTX26115 WB Image

WB analysis of rat IgG using GTX26115 Goat Anti-Rat IgG antibody, F(ab')2 fragment, pre-adsorbed (FITC).
Loading : 50 ng
Dilution : 1:1000

REFERENCE

Application Reference

Ho YJ et al. Biomaterials 2020; 232 : 119723 Superhydrophobic drug-loaded mesoporous silica nanoparticles capped with β-cyclodextrin for ultrasound image-guided combined antivascular and chemo-sonodynamic therapy.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 179