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Renilla Luciferase antibody

Anti-Renilla Luciferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125852
Anti-Renilla Luciferase antibody used in Western Blot (WB). GTX125852

Cat. No. GTX125852

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T transfected Renilla Luciferase

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the center region of Renilla Luciferase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172948

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Renilla Luciferase , Luciferase

Research Area

DATA IMAGES

Anti-Renilla Luciferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125852

GTX125852 ICC/IF Image

Renilla Luciferase antibody detects Renilla Luciferase protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Renilla Luciferase stained by Renilla Luciferase antibody (GTX125852) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Renilla Luciferase antibody used in Western Blot (WB). GTX125852

GTX125852 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: 293T transfected firefly
C: 293T transfected Renilla Luciferase
10% SDS PAGE
GTX125852 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169