APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Zebrafish, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding acetyl Lys68 of human SOD2. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
superoxide dismutase 2 , IPO-B , IPOB , MNSOD , MVCD6 , Mn-SOD
Cellular Localization
Mitochondrion matrix
Background
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]
Database
Research Area
DATA IMAGES
![Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Western Blot (WB). GTX636934](/templates/default/js/lightbox/images/loading.gif "Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Western Blot (WB). GTX636934")
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GTX636934 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636934 WB Image
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636934 Dot Image
Dotblot analysis of anti-SOD2 (Acetyl Lys68) antibody [HL1460] with peptide samples. Peptide samples were spotted onto positively charged nylon membrane and blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) varied dilution.
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REFERENCE
There are currently no references for SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934). Be the first to share your experience with this product.
![Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Western Blot (WB). GTX636934](/upload/website/prouct_img/small/GTX636934/GTX636934_44788_20220902_WB_B_22092119_512.webp "Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Western Blot (WB). GTX636934")
![Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Western Blot (WB). GTX636934](/upload/website/prouct_img/small/GTX636934/GTX636934_44788_20221007_WB_M_heart_22101319_444.webp "Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Western Blot (WB). GTX636934")
![Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Dot blot (Dot). GTX636934](/upload/website/prouct_img/small/GTX636934/GTX636934_T-44662_20240422_Dot_24043002_612.webp "Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Dot blot (Dot). GTX636934")
![Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Western Blot (WB). GTX636934](/upload/website/prouct_img/normal/GTX636934/GTX636934_44788_20220902_WB_B_22092119_512.webp "Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Western Blot (WB). GTX636934")
![Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Western Blot (WB). GTX636934](/upload/website/prouct_img/normal/GTX636934/GTX636934_44788_20221007_WB_M_heart_22101319_444.webp "Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Western Blot (WB). GTX636934")
![Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Dot blot (Dot). GTX636934](/upload/website/prouct_img/normal/GTX636934/GTX636934_T-44662_20240422_Dot_24043002_612.webp "Anti-SOD2 (Acetyl Lys68) antibody [HL1460] used in Dot blot (Dot). GTX636934")