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SP1 antibody [HL1396]

Recombinant antibody KO/KD Overexpression
Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836
Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
Anti-SP1 antibody [HL1396] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636836
Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836
Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836
Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836
Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836

Cat. No. GTX636836

Reference 2

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1396

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Product Note

This antibody is specific for human SP1 protein, and it does not cross react with human SP2, SP3, and SP4 protein.

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Sp1 transcription factor

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836

GTX636836 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836

GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836

GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836

GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SP1 antibody [HL1396] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636836

GTX636836 ICC/IF Image

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836

GTX636836 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836

GTX636836 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836

GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836

GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Fosso E et al. Antioxidants (Basel) 2023; 12 (8) Quercetin's Dual Mode of Action to Counteract the Sp1-miR-27a Axis in Colorectal Cancer Cells.
Emanuele Fosso et al. Antioxidants 2023; 12 (8) : 1547 Quercetin’s Dual Mode of Action to Counteract the Sp1-miR-27a Axis in Colorectal Cancer Cells
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations